Anti Phospho-FOXO3 (S253) mAb (Clone 1E1)

CUSABIO

Catalog No.:
CSB-RA008836A253phHU-50
Western Blot<br /> Positive WB detected in Hela whole cell lysate锛?93 whole cell lysate锛孉549 whole cell lysate<br /> All lanes Phospho-FOXO3 antibody at 0.96μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 97 KDa<br /> Observed band size: 97 KDa<br />
IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 10)

Documents & Links for Anti Phospho-FOXO3 (S253) mAb (Clone 1E1)
Datasheet Anti Phospho-FOXO3 (S253) mAb (Clone 1E1) Datasheet

Documents & Links for Anti Phospho-FOXO3 (S253) mAb (Clone 1E1)
Datasheet Anti Phospho-FOXO3 (S253) mAb (Clone 1E1) Datasheet
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