Anti Phospho-EIF4E (S209) mAb (Clone 1F3)

CUSABIO

SKU:
CSB-RA007556A209phHU-50
  • Western Blot<br />
Positive WB detected in 293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not)<br />
All lanes Phospho-EIF4E antibody at 1.2μg/ml<br />
Secondary<br />
Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
Predicted band size: 25 KDa<br />
Observed band size: 25 KDa<br />
  • IHC image of CSB-RA007556A209phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1F3)


Documents & Links for Anti Phospho-EIF4E (S209) mAb (Clone 1F3)
Datasheet Anti Phospho-EIF4E (S209) mAb (Clone 1F3) Datasheet

Documents & Links for Anti Phospho-EIF4E (S209) mAb (Clone 1F3)
Datasheet Anti Phospho-EIF4E (S209) mAb (Clone 1F3) Datasheet