Anti Phospho-EIF2S1 (S51) mAb (Clone 1C6)

CUSABIO

Catalog No.:
CSB-RA007523A51phHU-50
Western Blot<br /> Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF) <br /> All lanes Phospho-EIF2S1 antibody at 1.48μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 36 KDa<br /> Observed band size: 36 KDa<br />
IHC image of CSB-RA007523A51phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA007523A51phHU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells(treated with 100ng/ml EGF for 20min) with CSB-RA007523A51phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1C6)

Documents & Links for Anti Phospho-EIF2S1 (S51) mAb (Clone 1C6)
Datasheet Anti Phospho-EIF2S1 (S51) mAb (Clone 1C6) Datasheet

Documents & Links for Anti Phospho-EIF2S1 (S51) mAb (Clone 1C6)
Datasheet Anti Phospho-EIF2S1 (S51) mAb (Clone 1C6) Datasheet
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