Anti Phospho-CREB1 (S133) mAb (Clone 3C11)

CUSABIO

Catalog No.:
CSB-RA005947A133phHU-50
Western Blot<br /> Positive WB detected in Hela whole cell lysate,293 whole cell lysate<br /> All lanes Phospho-CREB1 antibody at 1.65μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 46 KDa<br /> Observed band size: 46 KDa<br />
IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA005947A133phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 3C11)

Documents & Links for Anti Phospho-CREB1 (S133) mAb (Clone 3C11)
Datasheet Anti Phospho-CREB1 (S133) mAb (Clone 3C11) Datasheet

Documents & Links for Anti Phospho-CREB1 (S133) mAb (Clone 3C11)
Datasheet Anti Phospho-CREB1 (S133) mAb (Clone 3C11) Datasheet
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