Anti Phospho-CDK2 (Y15) mAb (Clone 2C4)

CUSABIO

SKU:
CSB-RA005061A15phHU-50
  • Western Blot<br />Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not)<br />All lanes:Phospho-CDK2 antibody at 0.8μg/ml<br />Secondary<br />Goat polyclonal to rabbit IgG at 1/50000 dilution<br />Predicted band size: 34 KDa<br />Observed band size: 34 KDa<br />
  • Immunocytochemistry analysis of CSB-RA005061A15phHU diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate<br />Lane 1: Rabbit control IgG(1μg)instead of CSB-RA005061A15phHU in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />Lane 2: CSB-RA005061A15phHU(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)<br />Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)<br />
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Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431).

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2C4)


Documents & Links for Anti Phospho-CDK2 (Y15) mAb (Clone 2C4)
Datasheet Anti Phospho-CDK2 (Y15) mAb (Clone 2C4) Datasheet
Vendor Page Anti Phospho-CDK2 (Y15) mAb (Clone 2C4) at CUSABIO

Documents & Links for Anti Phospho-CDK2 (Y15) mAb (Clone 2C4)
Datasheet Anti Phospho-CDK2 (Y15) mAb (Clone 2C4) Datasheet
Vendor Page Anti Phospho-CDK2 (Y15) mAb (Clone 2C4)