Anti PABPN1 mAb (Clone 6C3)

CUSABIO

Catalog No.:
CSB-RA569290A0HU-50
Western Blot<br /> Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate<br /> All lanes: PABPN1 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 33, 32, 38 kDa<br /> Observed band size: 50 kDa<br />
IHC image of CSB-RA569290A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA569290A0HU diluted at 1:100 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA569290A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Shipping:
Calculated at Checkout
$221.00
Adding to cart… The item has been added
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity).

Product Specifications
Application WB, IHC, IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 6C3)

Documents & Links for Anti PABPN1 mAb (Clone 6C3)
Datasheet Anti PABPN1 mAb (Clone 6C3) Datasheet

Documents & Links for Anti PABPN1 mAb (Clone 6C3)
Datasheet Anti PABPN1 mAb (Clone 6C3) Datasheet
!