Anti NUP98 mAb (Clone 2H4)

CUSABIO

Catalog No.:
CSB-RA995655A0HU-50
Western Blot<br /> Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate<br /> All lanes: NUP98 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 198, 188, 98, 97, 196, 187 kDa<br /> Observed band size: 100 kDa<br />
IHC image of CSB-RA995655A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA995655A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2H4)

Documents & Links for Anti NUP98 mAb (Clone 2H4)
Datasheet Anti NUP98 mAb (Clone 2H4) Datasheet

Documents & Links for Anti NUP98 mAb (Clone 2H4)
Datasheet Anti NUP98 mAb (Clone 2H4) Datasheet
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