Anti-Nucleolin (Human) mAb (Clone: 4E2)

MBL Life Sciences

Catalog No.: MBL-M019-3

Monoclonal Antibody of 100 μg targeting Nucleolin for FCM, ICC, IHC, WB.

Product Specifications

• Application: FC, ICC, WB, IHC
• Reactivity: Human, Canine, Monkey, Bovine
• Clonality: Monoclonal IgG1
• Host: Mouse
• Immunogen: human nucleolin from Raji cell extract
• Gene ID - Human:

  4691

• Gene ID - Mouse:

  17975

• Formulation: 100 μg IgG in 100 μl PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
• Storage: -20C

Citations for Anti-Nucleolin (Human) mAb (Clone: 4E2) – 33 Found

Takagi, M; Matsuoka, Y; Kurihara, T; Yoneda, Y. Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. Journal Of Cell Science. 1999;112 ( Pt 15):2463-72. 
- Comments: Used in: ICC

Bates, P J; Kahlon, J B; Thomas, S D; Trent, J O; Miller, D M. Antiproliferative activity of G-rich oligonucleotides correlates with protein binding. The Journal Of Biological Chemistry. 1999;274(37):26369-77. 
- Comments: Used in: WB

Morimoto, Hiroyuki; Okamura, Hirohiko; Haneji, Tatsuji. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. The Journal Of Histochemistry And Cytochemistry : Official Journal Of The Histochemistry Society. 2002;50(9):1187-93. 
- Comments: Used in: WB

Morimoto, Hiroyuki; Okamura, Hirohiko; Haneji, Tatsuji. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. The Journal Of Histochemistry And Cytochemistry : Official Journal Of The Histochemistry Society. 2002;50(9):1187-93. 
- Comments: Used in: IP

Morimoto, Hiroyuki; Okamura, Hirohiko; Haneji, Tatsuji. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. The Journal Of Histochemistry And Cytochemistry : Official Journal Of The Histochemistry Society. 2002;50(9):1187-93. 
- Comments: Used in: ICC

Kumar, Vivek; Farell, Gerard; Deganello, Sergio; Lieske, John C. Annexin II is present on renal epithelial cells and binds calcium oxalate monohydrate crystals. Journal Of The American Society Of Nephrology : Jasn. 2003;14(2):289-97. 

Guo, Yan Xiang; Dallmann, Klara; Kwang, Jimmy. Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha. Virology. 2003;306(2):225-35. 
- Comments: Used in: ICC

Watson, Walter H; Jones, Dean P. Oxidation of nuclear thioredoxin during oxidative stress. Febs Letters. 2003;543(1-3):144-7. 
- Comments: Used in: WB

Barel, Monique; Balbo, Michelle; Le Romancer, Muriel; Frade, Raymond. Activation of Epstein-Barr virus/C3d receptor (gp140, CR2, CD21) on human cell surface triggers pp60src and Akt-GSK3 activities upstream and downstream to PI 3-kinase, respectively. European Journal Of Immunology. 2003;33(9):2557-66. 
- Comments: Used in: WB

Barel, Monique; Balbo, Michelle; Le Romancer, Muriel; Frade, Raymond. Activation of Epstein-Barr virus/C3d receptor (gp140, CR2, CD21) on human cell surface triggers pp60src and Akt-GSK3 activities upstream and downstream to PI 3-kinase, respectively. European Journal Of Immunology. 2003;33(9):2557-66. 
- Comments: Used in: IP

Baran, Vladimir; Pavlok, Antonin; Bjerregaard, Bolette; Wrenzycki, Christine; Hermann, Doris; Philimonenko, Vlada V; Lapathitis, Georgios; Hozak, Pavel; Niemann, Heiner; Motlik, Jan. Immunolocalization of upstream binding factor and pocket protein p130 during final stages of bovine oocyte growth. Biology Of Reproduction. 2004;70(4):877-86. 
- Comments: Used in: ICC

Gueven, Nuri; Becherel, Olivier J; Kijas, Amanda W; Chen, Philip; Howe, Orla; Rudolph, Jeanette H; Gatti, Richard; Date, Hidetoshi; Onodera, Osamu; Taucher-Scholz, Gisela; Lavin, Martin F. Aprataxin, a novel protein that protects against genotoxic stress. Human Molecular Genetics. 2004;13(10):1081-93. 
- Comments: Used in: WB

Gueven, Nuri; Becherel, Olivier J; Kijas, Amanda W; Chen, Philip; Howe, Orla; Rudolph, Jeanette H; Gatti, Richard; Date, Hidetoshi; Onodera, Osamu; Taucher-Scholz, Gisela; Lavin, Martin F. Aprataxin, a novel protein that protects against genotoxic stress. Human Molecular Genetics. 2004;13(10):1081-93. 
- Comments: Used in: ICC

Kumar, Vivek; Yu, Shihui; Farell, Gerard; Toback, F Gary; Lieske, John C. Renal epithelial cells constitutively produce a protein that blocks adhesion of crystals to their surface. American Journal Of Physiology. Renal Physiology. 2004;287(3):F373-83. 
- Comments: Used in: ICC

Bose, Santanu; Basu, Mausumi; Banerjee, Amiya K. Role of nucleolin in human parainfluenza virus type 3 infection of human lung epithelial cells. Journal Of Virology. 2004;78(15):8146-58. 
- Comments: Used in: WB

Sorokina, Elena A; Wesson, Jeffrey A; Kleinman, Jack G. An acidic peptide sequence of nucleolin-related protein can mediate the attachment of calcium oxalate to renal tubule cells. Journal Of The American Society Of Nephrology : Jasn. 2004;15(8):2057-65. 
- Comments: Used in: WB

Sorokina, Elena A; Wesson, Jeffrey A; Kleinman, Jack G. An acidic peptide sequence of nucleolin-related protein can mediate the attachment of calcium oxalate to renal tubule cells. Journal Of The American Society Of Nephrology : Jasn. 2004;15(8):2057-65. 
- Comments: Used in: ICC

Kawauchi, Kiyotaka; Ihjima, Kimiko; Yamada, Osamu. IL-2 increases human telomerase reverse transcriptase activity transcriptionally and posttranslationally through phosphatidylinositol 3'-kinase/Akt, heat shock protein 90, and mammalian target of rapamycin in transformed NK cells. Journal Of Immunology (Baltimore, Md. : 1950). 2005;174(9):5261-9. 
- Comments: Used in: WB

Sakaguchi, Masakiyo; Sonegawa, Hiroyuki; Nukui, Takamasa; Sakaguchi, Yoshihiko; Miyazaki, Masahiro; Namba, Masayoshi; Huh, Nam-Ho. Bifurcated converging pathways for high Ca2+- and TGFbeta-induced inhibition of growth of normal human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2005;102(39):13921-6. 
- Comments: Used in: WB

Wu, Ying-Li; Dudognon, Charles; Nguyen, Eric; Hillion, Josette; Pendino, Frédéric; Tarkanyi, Ilona; Aradi, Janos; Lanotte, Michel; Tong, Jian-Hua; Chen, Guo-Qiang; Ségal-Bendirdjian, Evelyne. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. Journal Of Cell Science. 2006;119(Pt 13):2797-806. 
- Comments: Used in: WB

Wu, Ying-Li; Dudognon, Charles; Nguyen, Eric; Hillion, Josette; Pendino, Frédéric; Tarkanyi, Ilona; Aradi, Janos; Lanotte, Michel; Tong, Jian-Hua; Chen, Guo-Qiang; Ségal-Bendirdjian, Evelyne. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. Journal Of Cell Science. 2006;119(Pt 13):2797-806. 
- Comments: Used in: IP

Becherel, Olivier J; Gueven, Nuri; Birrell, Geoff W; Schreiber, Valérie; Suraweera, Amila; Jakob, Burkhard; Taucher-Scholz, Gisela; Lavin, Martin F. Nucleolar localization of aprataxin is dependent on interaction with nucleolin and on active ribosomal DNA transcription. Human Molecular Genetics. 2006;15(14):2239-49. 
- Comments: Used in: WB

Becherel, Olivier J; Gueven, Nuri; Birrell, Geoff W; Schreiber, Valérie; Suraweera, Amila; Jakob, Burkhard; Taucher-Scholz, Gisela; Lavin, Martin F. Nucleolar localization of aprataxin is dependent on interaction with nucleolin and on active ribosomal DNA transcription. Human Molecular Genetics. 2006;15(14):2239-49. 
- Comments: Used in: ICC

Shiohama, Aiko; Sasaki, Takashi; Noda, Setsuko; Minoshima, Shinsei; Shimizu, Nobuyoshi. Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins. Experimental Cell Research. 2007;313(20):4196-207. 
- Comments: Used in: WB

Kitao, Saori; Segref, Alexandra; Kast, Juergen; Wilm, Matthias; Mattaj, Iain W; Ohno, Mutsuhito. A compartmentalized phosphorylation/dephosphorylation system that regulates U snRNA export from the nucleus. Molecular And Cellular Biology. 2008;28(1):487-97. 
- Comments: Used in: WB

Zhu, Chun-Hong; Kim, Jinyong; Shay, Jerry W; Wright, Woodring E. SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport. Plos One. 3(11):e3716. 
- Comments: Used in: ICC

Osawa, Naoshi; Onoda, Naoyoshi; Kawajiri, Hidemi; Tezuka, Kenji; Takashima, Tsutomu; Ishikawa, Tetsuro; Miyauchi, Akira; Hirokawa, Mitsuyoshi; Wakasa, Kenichi; Hirakawa, Kosei. Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT. International Journal Of Molecular Medicine. 2009;24(6):733-41. 
- Comments: Used in: IHC

Gorsler, Theresa; Murzik, Ulrike; Ulbricht, Tobias; Hentschel, Julia; Hemmerich, Peter; Melle, Christian. DNA damage-induced translocation of S100A11 into the nucleus regulates cell proliferation. Bmc Cell Biology. 2010;11:100. 
- Comments: Used in: ICC

Pickering, Brian F; Yu, Dihua; Van Dyke, Michael W. Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16. The Journal Of Biological Chemistry. 2011;286(51):44095-44103. 
- Comments: Used in: WB

Uozumi, Naoki; Matsumoto, Hotaru; Saitoh, Hisato. Detection of O-propargyl-puromycin with SUMO and ubiquitin by click chemistry at PML-nuclear bodies during abortive proteasome activities. Biochemical And Biophysical Research Communications. 2016;474(2):247-251. 
- Comments: Used in: ICC

Kadono, Moe; Kanai, Akinori; Nagamachi, Akiko; Shinriki, Satoru; Kawata, Jin; Iwato, Koji; Kyo, Taiichi; Oshima, Kumi; Yokoyama, Akihiko; Kawamura, Takeshi; Nagase, Reina; Inoue, Daichi; Kitamura, Toshio; Inaba, Toshiya; Ichinohe, Tatsuo; Matsui, Hirotaka. Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia. Experimental Hematology. 2016;44(8):745-754.e4. 
- Comments: Used in: ICC

Hayashi, Yuki; Kato, Kazashi; Kimura, Keiji. The hierarchical structure of the perichromosomal layer comprises Ki67, ribosomal RNAs, and nucleolar proteins. Biochemical And Biophysical Research Communications. 2017;493(2):1043-1049. 
- Comments: Used in: WB

Hayashi, Yuki; Fujimura, Akiko; Kato, Kazashi; Udagawa, Rina; Hirota, Toru; Kimura, Keiji. Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Science Advances. 2018;4(6):eaap7777. 
- Comments: Used in: ICC