Anti-Multi Ubiquitin mAb (Clone: FK2)

MBL Life Sciences

Catalog No.: MBL-D058-3

Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules. Monoclonal antibody of 100 μg targeting Ubiquitin for WB.

Product Specifications

• Application: WB
• Reactivity: Human, Mouse, Monkey, Fruit fly, Yeast
• Clonality: Monoclonal IgG1
• Host: Mouse
• Immunogen: Lysozyme - poly-ubiquitin was purified crude
• Formulation: 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.

Citations for Anti-Multi Ubiquitin mAb (Clone: FK2) – 43 Found

Yokosawa, Noriko; Yokota, Shin-Ichi; Kubota, Toru; Fujii, Nobuhiro. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. Journal Of Virology. 2002;76(24):12683-90. 
- Comments: Used in: WB

Yokosawa, Noriko; Yokota, Shin-Ichi; Kubota, Toru; Fujii, Nobuhiro. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. Journal Of Virology. 2002;76(24):12683-90. 
- Comments: Used in: ICC

Seino, Hiroaki; Kishi, Tsutomu; Nishitani, Hideo; Yamao, Fumiaki. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Molecular And Cellular Biology. 2003;23(10):3497-505. 
- Comments: Used in: WB

Katoh, Keiichi; Shibata, Hideki; Suzuki, Hidenori; Nara, Atsuki; Ishidoh, Kazumi; Kominami, Eiki; Yoshimori, Tamotsu; Maki, Masatoshi. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. The Journal Of Biological Chemistry. 2003;278(40):39104-13. 
- Comments: Used in: ICC

Inukai, Naoto; Yamaguchi, Yuki; Kuraoka, Isao; Yamada, Tomoko; Kamijo, Sachiko; Kato, Junko; Tanaka, Kiyoji; Handa, Hiroshi. A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. The Journal Of Biological Chemistry. 2004;279(9):8190-5. 
- Comments: Used in: WB

Broering, Teresa J; Arnold, Michelle M; Miller, Cathy L; Hurt, Jessica A; Joyce, Patricia L; Nibert, Max L. Carboxyl-proximal regions of reovirus nonstructural protein muNS necessary and sufficient for forming factory-like inclusions. Journal Of Virology. 2005;79(10):6194-206. 
- Comments: Used in: ICC

Yamashita, Masakatsu; Shinnakasu, Ryo; Asou, Hikari; Kimura, Motoko; Hasegawa, Akihiro; Hashimoto, Kahoko; Hatano, Naoya; Ogata, Masato; Nakayama, Toshinori. Ras-ERK MAPK cascade regulates GATA3 stability and Th2 differentiation through ubiquitin-proteasome pathway. The Journal Of Biological Chemistry. 2005;280(33):29409-19. 
- Comments: Used in: WB

Hwang, Gi-Wook; Sasaki, Daisuke; Naganuma, Akira. Overexpression of Rad23 confers resistance to methylmercury in saccharomyces cerevisiae via inhibition of the degradation of ubiquitinated proteins. Molecular Pharmacology. 2005;68(4):1074-8. 
- Comments: Used in: WB

Masuda, Yutaka; Itabe, Hiroyuki; Odaki, Miho; Hama, Kotaro; Fujimoto, Yasuyuki; Mori, Masahiro; Sasabe, Naoko; Aoki, Junken; Arai, Hiroyuki; Takano, Tatsuya. ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells. Journal Of Lipid Research. 2006;47(1):87-98. 
- Comments: Used in: WB

Nakamura, Michihiko; Tanaka, Nobuyuki; Kitamura, Naomi; Komada, Masayuki. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes. Genes To Cells : Devoted To Molecular & Cellular Mechanisms. 2006;11(6):593-606. 
- Comments: Used in: ICC

Hosokawa, Hiroyuki; Kimura, Motoko Y; Shinnakasu, Ryo; Suzuki, Akane; Miki, Takako; Koseki, Haruhiko; van Lohuizen, Maarten; Yamashita, Masakatsu; Nakayama, Toshinori. Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3. Journal Of Immunology (Baltimore, Md. : 1950). 2006;177(11):7656-64. 
- Comments: Used in: WB

Ishioka, Toshiyasu; Katayama, Ryohei; Kikuchi, Ryo; Nishimoto, Michie; Takada, Shinji; Takada, Ritsuko; Matsuzawa, Shu-ichi; Reed, John C; Tsuruo, Takashi; Naito, Mikihiko. Impairment of the ubiquitin-proteasome system by cellular FLIP. Genes To Cells : Devoted To Molecular & Cellular Mechanisms. 2007;12(6):735-44. 
- Comments: Used in: ICC

Komatsu, Masaaki; Waguri, Satoshi; Koike, Masato; Sou, Yu-Shin; Ueno, Takashi; Hara, Taichi; Mizushima, Noboru; Iwata, Jun-Ichi; Ezaki, Junji; Murata, Shigeo; Hamazaki, Jun; Nishito, Yasumasa; Iemura, Shun-Ichiro; Natsume, Tohru; Yanagawa, Toru; Uwayama, Junya; Warabi, Eiji; Yoshida, Hiroshi; Ishii, Tetsuro; Kobayashi, Akira; Yamamoto, Masayuki; Yue, Zhenyu; Uchiyama, Yasuo; Kominami, Eiki; Tanaka, Keiji. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 2007;131(6):1149-63. 
- Comments: Used in: WB

Komatsu, Masaaki; Waguri, Satoshi; Koike, Masato; Sou, Yu-Shin; Ueno, Takashi; Hara, Taichi; Mizushima, Noboru; Iwata, Jun-Ichi; Ezaki, Junji; Murata, Shigeo; Hamazaki, Jun; Nishito, Yasumasa; Iemura, Shun-Ichiro; Natsume, Tohru; Yanagawa, Toru; Uwayama, Junya; Warabi, Eiji; Yoshida, Hiroshi; Ishii, Tetsuro; Kobayashi, Akira; Yamamoto, Masayuki; Yue, Zhenyu; Uchiyama, Yasuo; Kominami, Eiki; Tanaka, Keiji. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 2007;131(6):1149-63. 
- Comments: Used in: ICC

Shi, Wei; Ma, Zhefu; Willers, Henning; Akhtar, Kamal; Scott, Shaun P; Zhang, Jiuqin; Powell, Simon; Zhang, Junran. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. The Journal Of Biological Chemistry. 2008;283(46):31608-16. 
- Comments: Used in: WB

Shi, Wei; Ma, Zhefu; Willers, Henning; Akhtar, Kamal; Scott, Shaun P; Zhang, Jiuqin; Powell, Simon; Zhang, Junran. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. The Journal Of Biological Chemistry. 2008;283(46):31608-16. 
- Comments: Used in: ICC

Gitcho, Michael A; Strider, Jeffrey; Carter, Deborah; Taylor-Reinwald, Lisa; Forman, Mark S; Goate, Alison M; Cairns, Nigel J. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. The Journal Of Biological Chemistry. 2009;284(18):12384-98. 
- Comments: Used in: WB

Gitcho, Michael A; Strider, Jeffrey; Carter, Deborah; Taylor-Reinwald, Lisa; Forman, Mark S; Goate, Alison M; Cairns, Nigel J. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. The Journal Of Biological Chemistry. 2009;284(18):12384-98. 
- Comments: Used in: ICC

Ebina, Manato; Tsuruta, Fuminori; Katoh, Megumi C; Kigoshi, Yu; Someya, Akie; Chiba, Tomoki. Myeloma overexpressed 2 (Myeov2) regulates L11 subnuclear localization through Nedd8 modification. Plos One. 8(6):e65285. 
- Comments: Used in: WB

Furuya, Norihiko; Ikeda, Shin-Ichi; Sato, Shigeto; Soma, Sanae; Ezaki, Junji; Oliva Trejo, Juan Alejandro; Takeda-Ezaki, Mitsue; Fujimura, Tsutomu; Arikawa-Hirasawa, Eri; Tada, Norihiro; Komatsu, Masaaki; Tanaka, Keiji; Kominami, Eiki; Hattori, Nobutaka; Ueno, Takashi. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy. 2014;10(4):631-41. 
- Comments: Used in: WB

Ogawa, Mitsutaka; Sawaguchi, Shogo; Kawai, Takami; Nadano, Daita; Matsuda, Tsukasa; Yagi, Hirokazu; Kato, Koichi; Furukawa, Koichi; Okajima, Tetsuya. Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine transferase found in Adams-Oliver syndrome. The Journal Of Biological Chemistry. 2015;290(4):2137-49. 
- Comments: Used in: WB

Choi, Un Yung; Choi, Won Young; Hur, Ji Yeon; Kim, Young-Joon. Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies. Experimental & Molecular Medicine. 2015;47:e159. 
- Comments: Used in: ICC

Sin, Yooksil; Tanaka, Kiyoji; Saijo, Masafumi. The C-terminal Region and SUMOylation of Cockayne Syndrome Group B Protein Play Critical Roles in Transcription-coupled Nucleotide Excision Repair. The Journal Of Biological Chemistry. 2016;291(3):1387-97. 
- Comments: Used in: WB

Ohoka, Nobumichi; Nagai, Katsunori; Shibata, Norihito; Hattori, Takayuki; Nara, Hiroshi; Cho, Nobuo; Naito, Mikihiko. SNIPER(TACC3) induces cytoplasmic vacuolization and sensitizes cancer cells to Bortezomib. Cancer Science. 2017;108(5):1032-1041. 
- Comments: Used in: ICC

Takayama, Kaori; Matsuura, Akira; Itakura, Eisuke. Dissection of ubiquitinated protein degradation by basal autophagy. Febs Letters. 2017;591(9):1199-1211. 
- Comments: Used in: WB

Takayama, Kaori; Matsuura, Akira; Itakura, Eisuke. Dissection of ubiquitinated protein degradation by basal autophagy. Febs Letters. 2017;591(9):1199-1211. 
- Comments: Used in: ICC

Okuda, Kosuke; Kobayashi, Shizuka; Fukaya, Masahiro; Watanabe, Aya; Murakami, Takuto; Hagiwara, Mai; Sato, Tempei; Ueno, Hiroe; Ogonuki, Narumi; Komano-Inoue, Sayaka; Manabe, Hiroyuki; Yamaguchi, Masahiro; Ogura, Atsuo; Asahara, Hiroshi; Sakagami, Hiroyuki; Mizuguchi, Masashi; Manabe, Toshiya; Tanaka, Teruyuki. CDKL5 controls postsynaptic localization of GluN2B-containing NMDA receptors in the hippocampus and regulates seizure susceptibility. Neurobiology Of Disease. 2017;106:158-170. 
- Comments: Used in: WB

Joachim, Justin; Razi, Minoo; Judith, Delphine; Wirth, Martina; Calamita, Emily; Encheva, Vesela; Dynlacht, Brian D; Snijders, Ambrosius P; O'Reilly, Nicola; Jefferies, Harold B J; Tooze, Sharon A. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Current Biology : Cb. 2017;27(14):2123-2136.e7. 
- Comments: Used in: WB

Kim, Jaehyun; Tsuruta, Fuminori; Okajima, Tomomi; Yano, Sarasa; Sato, Ban; Chiba, Tomoki. KLHL7 promotes TUT1 ubiquitination associated with nucleolar integrity: Implications for retinitis pigmentosa. Biochemical And Biophysical Research Communications. 2017;494(1-2):220-226. 
- Comments: Used in: WB

Tsuiji, Hitomi; Inoue, Ikuyo; Takeuchi, Mari; Furuya, Asako; Yamakage, Yuko; Watanabe, Seiji; Koike, Masato; Hattori, Mitsuharu; Yamanaka, Koji. TDP-43 accelerates age-dependent degeneration of interneurons. Scientific Reports. 2017;7(1):14972. 
- Comments: Used in: IHC

Watanabe, Seiji; Komine, Okiru; Endo, Fumito; Wakasugi, Keisuke; Yamanaka, Koji. Intracerebroventricular administration of Cystatin C ameliorates disease in SOD1-linked amyotrophic lateral sclerosis mice. Journal Of Neurochemistry. 2018;145(1):80-89. 
- Comments: Used in: IHC

Kang, Di; Wang, Dou; Xu, Jianbing; Quan, Chao; Guo, Xuan; Wang, Heng; Luo, Jun; Yang, Zhongzhou; Chen, Shuai; Chen, Jiong. The InR/Akt/TORC1 Growth-Promoting Signaling Negatively Regulates JAK/STAT Activity and Migratory Cell Fate during Morphogenesis. Developmental Cell. 2018;44(4):524-531.e5. 
- Comments: Used in: WB

Xie, Xuan; Matsumoto, Shunsuke; Endo, Akinori; Fukushima, Toshiaki; Kawahara, Hiroyuki; Saeki, Yasushi; Komada, Masayuki. Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities. Journal Of Cell Science. 2018;131(8) 
- Comments: Used in: ICC

Mitsuhashi, Hiroaki; Ishimaru, Satoshi; Homma, Sachiko; Yu, Bryant; Honma, Yuki; Beermann, Mary Lou; Miller, Jeffrey Boone. Functional domains of the FSHD-associated DUX4 protein. Biology Open. 2018;7(4) 
- Comments: Used in: WB

Matsumoto, Gen; Inobe, Tomonao; Amano, Takanori; Murai, Kiyohito; Nukina, Nobuyuki; Mori, Nozomu. N-Acyldopamine induces aggresome formation without proteasome inhibition and enhances protein aggregation via p62/SQSTM1 expression. Scientific Reports. 2018;8(1):9585. 
- Comments: Used in: WB

Jena, Kautilya Kumar; Kolapalli, Srinivasa Prasad; Mehto, Subhash; Nath, Parej; Das, Biswajit; Sahoo, Pradyumna Kumar; Ahad, Abdul; Syed, Gulam Hussain; Raghav, Sunil K; Senapati, Shantibhusan; Chauhan, Swati; Chauhan, Santosh. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. The Embo Journal. 2018;37(18) 
- Comments: Used in: WB

Cao, Zhengyi; Xue, Junhui; Cheng, Yuning; Wang, Jiyin; Liu, Yujuan; Li, Hui; Jiang, Wei; Li, Gang; Gui, Yaoting; Zhang, Xiaowei. MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. Oncogene. 2019;38(24):4835-4855. 
- Comments: Used in: WB

Chernyshova, Kseniia; Inoue, Keiichi; Yamashita, Shun-Ichi; Fukuchi, Takeo; Kanki, Tomotake. Glaucoma-Associated Mutations in the Optineurin Gene Have Limited Impact on Parkin-Dependent Mitophagy. Investigative Ophthalmology & Visual Science. 2019;60(10):3625-3635. 
- Comments: Used in: WB

Xue, Junhui; Cao, Zhengyi; Cheng, Yuning; Wang, Jiyin; Liu, Yujuan; Yang, Ruixiang; Li, Hui; Jiang, Wei; Li, Gang; Zhao, Wenhui; Zhang, Xiaowei. Acetylation of alpha-fetoprotein promotes hepatocellular carcinoma progression. Cancer Letters. 2020;471:12-26. 
- Comments: Used in: WB

Asahina, Makoto; Fujinawa, Reiko; Nakamura, Sayuri; Yokoyama, Kotaro; Tozawa, Ryuichi; Suzuki, Tadashi. Ngly1 -/- rats develop neurodegenerative phenotypes and pathological abnormalities in their peripheral and central nervous systems. Human Molecular Genetics. 2020;29(10):1635-1647. 
- Comments: Used in: WB

Tsuda, Masataka; Kitamasu, Kaito; Kumagai, Chiho; Sugiyama, Kazuya; Nakano, Toshiaki; Ide, Hiroshi. Tyrosyl-DNA phosphodiesterase 2 (TDP2) repairs topoisomerase 1 DNA-protein crosslinks and 3'-blocking lesions in the absence of tyrosyl-DNA phosphodiesterase 1 (TDP1). Dna Repair. 2020;91-92:102849. 
- Comments: Used in: WB

Nakai, Naoya; Kitai, Saki; Iida, Noriko; Inoue, Sachika; Higashida, Kazuhiko. Autophagy under glucose starvation enhances protein translation initiation in response to re-addition of glucose in C2C12 myotubes. Febs Open Bio. 2020;10(10):2149-2156. 
- Comments: Used in: WB

Sakai, Wataru; Yuasa-Sunagawa, Mayumi; Kusakabe, Masayuki; Kishimoto, Aiko; Matsui, Takeshi; Kaneko, Yuki; Akagi, Jun-Ichi; Huyghe, Nicolas; Ikura, Masae; Ikura, Tsuyoshi; Hanaoka, Fumio; Yokoi, Masayuki; Sugasawa, Kaoru. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair. Scientific Reports. 2020;10(1):19704. 
- Comments: Used in: WB