Anti MGMT mAb (Clone 10G7)

CUSABIO

Catalog No.:
CSB-RA966553A0HU-50
Western Blot<br /> Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate<br /> All lanes: MGMT antibody at 1:1500<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 22 kDa<br /> Observed band size: 22 kDa<br />
IHC image of CSB-RA966553A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 10G7)

Documents & Links for Anti MGMT mAb (Clone 10G7)
Datasheet Anti MGMT mAb (Clone 10G7) Datasheet

Documents & Links for Anti MGMT mAb (Clone 10G7)
Datasheet Anti MGMT mAb (Clone 10G7) Datasheet
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