Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.
Clonality: Monoclonal
Host: Mouse
Purification: Ig-PG
Reactivity: Mouse, Human
LC3 is a mammalian autophagosome marker. It is immediately cleaved to form LC3-I by Atg4, and when autophagy is induced, phosphatidylethanolamine is covalently linked to LC3-I C-terminal glycine to form LC3-II. LC3-II is membrane-bound and thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody shows good results in ICC and Immunoelectron microscopy experiments.
Source: Professor Noboru Mizushima, Department of Cell Physiology, Tokyo Medical and Dental University
References:
1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000)
2) Mizushima, N., Yoshimori, T. How to interpret LC3 immunoblotting Autophagy 3:542-545 (2007)
3) Mizushima, N., Yoshimori, T. and Levine, B. Methods in mammalian autophagy research. Cell 140; 313-326 (2010)
| Documents & Links for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) | |
| Datasheet | Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) Datasheet |
| Documents & Links for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) | |
| Datasheet | Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) Datasheet |
| Citations for Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3-1703) – 9 Found |
| Hee Lee et al. 2016. Inducing mitophagy in diabetic platelets protects against severe oxidative stress. EMBO Mol Med. 8(7):779-95. PubMed, Journal |
| Wijdeven et al. 2016. Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway. Nat Commun. 7(11808):. PubMed, Journal |
| Murase et al. 2015. Cooperation of endothelin-1 signaling with melanosomes plays a role in developing and/or maintaining human skin hyperpigmentation. Biology Open. 4:1213-21. PubMed, Journal |
| Yahiro et al. 2015. Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway. Cell Death Discov. 1(15035):. PubMed, Journal |
| Liang et al. 2015. Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication. J Virol. 89(10):5308-17. PubMed, Journal |
| Yahiro et al. 2014. DAP1, a negative regulator of autophagy, controls SubAB-mediated apoptosis and autophagy. Infect Immun. 82(11):4899-908. PubMed, Journal |
| Kishi-Itakura et al. 2014. Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells. J Cell Sci. 127(18):4089-102. PubMed, Journal |
| Jiang et al. 2014. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell. 25(8):1327-37. PubMed, Journal |
| Liang et al. 2014. Crosstalk between the cGAS DNA sensor and Beclin-1 autophagy protein shapes innate antimicrobial immune responses. Cell Host Microbe. 15(2):228-38. PubMed, Journal |