Anti-LC3 (Human) mAb (Clone: 4E12)

MBL Life Sciences

Catalog No.: MBL-M152-3

This LC3 antibody is validated for multiple applications (ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP. 

Product Specifications

• Application: FC, ICC, IP, IHC, Immuno-EM
• Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Monoclonal IgG1 ĸ
• Host: Mouse
• Source: Monoclonal
• Immunogen: Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)
• Formulation: 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Citations for Anti-LC3 (Human) mAb (Clone: 4E12) – 57 Found

Thayanidhi, Nandhakumar; Helm, Jared R; Nycz, Deborah C; Bentley, Marvin; Liang, Yingjian; Hay, Jesse C. Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs. Molecular Biology Of The Cell. 2010;21(11):1850-63. 
- Comments: Used in: ICC

Eng, Kai Er; Panas, Marc D; Karlsson Hedestam, Gunilla B; McInerney, Gerald M. A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 2010;6(5):634-41. 
- Comments: Used in: FCM

Saiki, Shinji; Sasazawa, Yukiko; Imamichi, Yoko; Kawajiri, Sumihiro; Fujimaki, Takahiro; Tanida, Isei; Kobayashi, Hiroki; Sato, Fumiaki; Sato, Shigeto; Ishikawa, Ken-Ichi; Imoto, Masaya; Hattori, Nobutaka. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 2011;7(2):176-87. 
- Comments: Used in: WB

Ho, Hsiang; Kapadia, Rubina; Al-Tahan, Sejad; Ahmad, Safoora; Ganesan, Anand K. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. The Journal Of Biological Chemistry. 2011;286(14):12509-23. 
- Comments: Used in: WB

Kovacs, J R; Li, C; Yang, Q; Li, G; Garcia, I G; Ju, S; Roodman, D G; Windle, J J; Zhang, X; Lu, B. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death And Differentiation. 2012;19(1):144-52. 
- Comments: Used in: ICC

Cai, T; Hirai, H; Zhang, G; Zhang, M; Takahashi, N; Kasai, H; Satin, L S; Leapman, R D; Notkins, A L. Deletion of Ia-2 and/or Ia-2β in mice decreases insulin secretion by reducing the number of dense core vesicles. Diabetologia. 2011;54(9):2347-57. 
- Comments: Used in: ICC

Traver, Maria K; Henry, Stanley C; Cantillana, Viviana; Oliver, Tim; Hunn, Julia P; Howard, Jonathan C; Beer, Sandra; Pfeffer, Klaus; Coers, Jörn; Taylor, Gregory A. Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. The Journal Of Biological Chemistry. 2011;286(35):30471-30480. 
- Comments: Used in: ICC

Moreau, Kevin; Ravikumar, Brinda; Renna, Maurizio; Puri, Claudia; Rubinsztein, David C. Autophagosome precursor maturation requires homotypic fusion. Cell. 2011;146(2):303-17. 
- Comments: Used in: ICC

Gillis, Judith M; Benckhuijsen, Willemien; van Veen, Henk; Sanz, Alicia Sanz; Drijfhout, Jan W; Reits, Eric A. Aminopeptidase-resistant peptides are targeted to lysosomes and subsequently degraded. Traffic (Copenhagen, Denmark). 2011;12(12):1897-910. 
- Comments: Used in: ICC

Frankel, Lisa B; Wen, Jiayu; Lees, Michael; Høyer-Hansen, Maria; Farkas, Thomas; Krogh, Anders; Jäättelä, Marja; Lund, Anders H. microRNA-101 is a potent inhibitor of autophagy. The Embo Journal. 2011;30(22):4628-41. 
- Comments: Used in: WB

Chalazonitis, Alcmène; Tang, Amy A; Shang, Yulei; Pham, Tuan D; Hsieh, Ivy; Setlik, Wanda; Gershon, Michael D; Huang, Eric J. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2011;31(39):13746-57. 
- Comments: Used in: IHC

Seto, Shintaro; Tsujimura, Kunio; Koide, Yukio. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cellular Microbiology. 2012;14(5):710-27. 
- Comments: Used in: ICC

Starr, Tregei; Child, Robert; Wehrly, Tara D; Hansen, Bryan; Hwang, Seungmin; López-Otin, Carlos; Virgin, Herbert W; Celli, Jean. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host & Microbe. 2012;11(1):33-45. 
- Comments: Used in: WB

Starr, Tregei; Child, Robert; Wehrly, Tara D; Hansen, Bryan; Hwang, Seungmin; López-Otin, Carlos; Virgin, Herbert W; Celli, Jean. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host & Microbe. 2012;11(1):33-45. 
- Comments: Used in: ICC

Shi, Chong-Shan; Shenderov, Kevin; Huang, Ning-Na; Kabat, Juraj; Abu-Asab, Mones; Fitzgerald, Katherine A; Sher, Alan; Kehrl, John H. Activation of autophagy by inflammatory signals limits IL-1β production by targeting ubiquitinated inflammasomes for destruction. Nature Immunology. 2012;13(3):255-63. 
- Comments: Used in: WB

Sims, Joshua J; Scavone, Francesco; Cooper, Eric M; Kane, Lesley A; Youle, Richard J; Boeke, Jef D; Cohen, Robert E. Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Nature Methods. 2012;9(3):303-9. 
- Comments: Used in: ICC

McKnight, Nicole C; Jefferies, Harold B J; Alemu, Endalkachew A; Saunders, Rebecca E; Howell, Michael; Johansen, Terje; Tooze, Sharon A. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. The Embo Journal. 2012;31(8):1931-46. 
- Comments: Used in: ICC

Morris, Chantey R; Stanton, Marissa J; Manthey, Karoline C; Oh, Keon Bong; Wagner, Kay-Uwe. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. Plos One. 7(3):e34308. 
- Comments: Used in: WB

Morris, Chantey R; Stanton, Marissa J; Manthey, Karoline C; Oh, Keon Bong; Wagner, Kay-Uwe. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. Plos One. 7(3):e34308. 
- Comments: Used in: ICC

Joubert, Pierre-Emmanuel; Werneke, Scott W; de la Calle, Claire; Guivel-Benhassine, Florence; Giodini, Alessandra; Peduto, Lucie; Levine, Beth; Schwartz, Olivier; Lenschow, Deborah J; Albert, Matthew L. Chikungunya virus-induced autophagy delays caspase-dependent cell death. The Journal Of Experimental Medicine. 2012;209(5):1029-47. 
- Comments: Used in: WB

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 
- Comments: Used in: WB

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 

Matsuzawa, Yu; Oshima, Shigeru; Takahara, Masahiro; Maeyashiki, Chiaki; Nemoto, Yasuhiro; Kobayashi, Masanori; Nibe, Yoichi; Nozaki, Kengo; Nagaishi, Takashi; Okamoto, Ryuichi; Tsuchiya, Kiichiro; Nakamura, Tetsuya; Ma, Averil; Watanabe, Mamoru. TNFAIP3 promotes survival of CD4 T cells by restricting MTOR and promoting autophagy. Autophagy. 11(7):1052-62. 
- Comments: Used in: ICC

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: WB

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: ICC

Silva, Bruno Jorge de Andrade; Barbosa, Mayara Garcia de Mattos; Andrade, Priscila Ribeiro; Ferreira, Helen; Nery, José Augusto da Costa; Côrte-Real, Suzana; da Silva, Gilberto Marcelo Sperandio; Rosa, Patricia Sammarco; Fabri, Mario; Sarno, Euzenir Nunes; Pinheiro, Roberta Olmo. Autophagy Is an Innate Mechanism Associated with Leprosy Polarization. Plos Pathogens. 2017;13(1):e1006103. 
- Comments: Used in: IHC

Abe, Shoko; Nagai, Tomoaki; Masukawa, Moe; Okumoto, Kanji; Homma, Yuta; Fujiki, Yukio; Mizuno, Kensaku. Localization of Protein Kinase NDR2 to Peroxisomes and Its Role in Ciliogenesis. The Journal Of Biological Chemistry. 2017;292(10):4089-4098. 
- Comments: Used in: ICC

Nakano, Satoshi; Oki, Mitsuaki; Kusaka, Hirofumi. The role of p62/SQSTM1 in sporadic inclusion body myositis. Neuromuscular Disorders : Nmd. 2017;27(4):363-369. 
- Comments: Used in: IHC

Ye, Mao; Qiu, Hong; Cao, Yingkang; Zhang, Min; Mi, Yan; Yu, Jing; Wang, Changhua. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Frontiers In Pharmacology. 8:148. 
- Comments: Used in: WB

Hasegawa, Haruki; Hsu, Ann; Tinberg, Christine E; Siegler, Karen E; Nazarian, Aaron A; Tsai, Mei-Mei. Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2α phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic. Mabs. 2017;9(5):854-873. 
- Comments: Used in: ICC

Arasaki, Kohei; Mikami, Yumi; Shames, Stephanie R; Inoue, Hiroki; Wakana, Yuichi; Tagaya, Mitsuo. Legionella effector Lpg1137 shuts down ER-mitochondria communication through cleavage of syntaxin 17. Nature Communications. 2017;8:15406. 
- Comments: Used in: ICC

Wacker, Rahel; Eickel, Nina; Schmuckli-Maurer, Jacqueline; Annoura, Takeshi; Niklaus, Livia; Khan, Shahid M; Guan, Jun-Lin; Heussler, Volker T. LC3-association with the parasitophorous vacuole membrane of Plasmodium berghei liver stages follows a noncanonical autophagy pathway. Cellular Microbiology. 2017;19(10) 
- Comments: Used in: ICC

Kimura, Tetsuya; Suzuki, Mamiko; Akagi, Takumi. Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus. Acta Neuropathologica Communications. 2017;5(1):67. 
- Comments: Used in: WB

Kimura, Tetsuya; Suzuki, Mamiko; Akagi, Takumi. Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus. Acta Neuropathologica Communications. 2017;5(1):67. 
- Comments: Used in: IHC

Zhao, Qiong; Hu, Zhan-Ying; Zhang, Jing-Pu; Jiang, Jian-Dong; Ma, Yuan-Yuan; Li, Jian-Rui; Peng, Zong-Gen; Chen, Jin-Hua. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Scientific Reports. 2017;7(1):11250. 
- Comments: Used in: ICC

Sato, Yusuke; Ohtsubo, Hideaki; Nihei, Naohiro; Kaneko, Takane; Sato, Yoriko; Adachi, Shin-Ichi; Kondo, Shinji; Nakamura, Mako; Mizunoya, Wataru; Iida, Hiroshi; Tatsumi, Ryuichi; Rada, Cristina; Yoshizawa, Fumiaki. Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2018;32(3):1428-1439. 
- Comments: Used in: IHC

Newton, Timothy; Allison, Rachel; Edgar, James R; Lumb, Jennifer H; Rodger, Catherine E; Manna, Paul T; Rizo, Tania; Kohl, Zacharias; Nygren, Anders O H; Arning, Larissa; Schüle, Rebecca; Depienne, Christel; Goldberg, Lisa; Frahm, Christiane; Stevanin, Giovanni; Durr, Alexandra; Schöls, Ludger; Winner, Beate; Beetz, Christian; Reid, Evan. Mechanistic basis of an epistatic interaction reducing age at onset in hereditary spastic paraplegia. Brain : A Journal Of Neurology. 2018;141(5):1286-1299. 
- Comments: Used in: ICC

Takahashi, Akiko; Loo, Tze Mun; Okada, Ryo; Kamachi, Fumitaka; Watanabe, Yoshihiro; Wakita, Masahiro; Watanabe, Sugiko; Kawamoto, Shimpei; Miyata, Kenichi; Barber, Glen N; Ohtani, Naoko; Hara, Eiji. Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells. Nature Communications. 2018;9(1):1249. 
- Comments: Used in: WB

Konno, Hiroyasu; Chinn, Ivan K; Hong, Diana; Orange, Jordan S; Lupski, James R; Mendoza, Alejandra; Pedroza, Luis A; Barber, Glen N. Pro-inflammation Associated with a Gain-of-Function Mutation (R284S) in the Innate Immune Sensor STING. Cell Reports. 2018;23(4):1112-1123. 
- Comments: Used in: ICC

Perot, Brieuc P; Boussier, Jeremy; Yatim, Nader; Rossman, Jeremy S; Ingersoll, Molly A; Albert, Matthew L. Autophagy diminishes the early interferon-β response to influenza A virus resulting in differential expression of interferon-stimulated genes. Cell Death & Disease. 2018;9(5):539. 

Wise, John P Jr; Price, Charles G; Amaro, Joseph A; Cannon, Jason R. Autophagy Disruptions Associated With Altered Optineurin Expression in Extranigral Regions in a Rotenone Model of Parkinson's Disease. Frontiers In Neuroscience. 12:289. 
- Comments: Used in: ICC

Kanda, Ranka; Hiraike, Haruko; Wada-Hiraike, Osamu; Ichinose, Takayuki; Nagasaka, Kazunori; Sasajima, Yuko; Ryo, Eiji; Fujii, Tomoyuki; Osuga, Yutaka; Ayabe, Takuya. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. Bmc Cancer. 2018;18(1):657. 
- Comments: Used in: WB

Kanda, Ranka; Hiraike, Haruko; Wada-Hiraike, Osamu; Ichinose, Takayuki; Nagasaka, Kazunori; Sasajima, Yuko; Ryo, Eiji; Fujii, Tomoyuki; Osuga, Yutaka; Ayabe, Takuya. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. Bmc Cancer. 2018;18(1):657. 
- Comments: Used in: ICC

Mori, Hiroyuki; Fukuhara, Takasuke; Ono, Chikako; Tamura, Tomokazu; Sato, Asuka; Fauzyah, Yuzy; Wada, Masami; Okamoto, Toru; Noda, Takeshi; Yoshimori, Tamotsu; Matsuura, Yoshiharu. Induction of selective autophagy in cells replicating hepatitis C virus genome. The Journal Of General Virology. 2018;99(12):1643-1657. 
- Comments: Used in: ICC

Kajiume, Teruyuki; Kobayashi, Masao. Human granulocytes undergo cell death via autophagy. Cell Death Discovery. 4:111. 
- Comments: Used in: ICC

Zemirli, Naïma; Boukhalfa, Asma; Dupont, Nicolas; Botti, Joëlle; Codogno, Patrice; Morel, Etienne. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 2019;3(3):100-109. 
- Comments: Used in: IF

Hou, Bolin; Wang, Gang; Gao, Quan; Wei, Yanjie; Zhang, Caining; Wang, Yange; Huo, Yuqing; Yang, Huaiyi; Jiang, Xuejun; Xi, Zhijun. SQSTM1/p62 loss reverses the inhibitory effect of sunitinib on autophagy independent of AMPK signaling. Scientific Reports. 2019;9(1):11087. 
- Comments: Used in: IP

Bordi, Matteo; Darji, Sandipkumar; Sato, Yutaka; Mellén, Marian; Berg, Martin J; Kumar, Asok; Jiang, Ying; Nixon, Ralph A. mTOR hyperactivation in Down Syndrome underlies deficits in autophagy induction, autophagosome formation, and mitophagy. Cell Death & Disease. 2019;10(8):563. 
- Comments: Used in: ICC

Lasič, Eva; Lisjak, Marjeta; Horvat, Anemari; Božić, Mićo; Šakanović, Aleksandra; Anderluh, Gregor; Verkhratsky, Alexei; Vardjan, Nina; Jorgačevski, Jernej; Stenovec, Matjaž; Zorec, Robert. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Scientific Reports. 2019;9(1):10957. 
- Comments: Used in: ICC

Vrahnas, Christina; Blank, Martha; Dite, Toby A; Tatarczuch, Liliana; Ansari, Niloufar; Crimeen-Irwin, Blessing; Nguyen, Huynh; Forwood, Mark R; Hu, Yifang; Ikegame, Mika; Bambery, Keith R; Petibois, Cyril; Mackie, Eleanor J; Tobin, Mark J; Smyth, Gordon K; Oakhill, Jonathan S; Martin, T John; Sims, Natalie A. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nature Communications. 2019;10(1):5073. 
- Comments: Used in: ICC

Vrahnas, Christina; Blank, Martha; Dite, Toby A; Tatarczuch, Liliana; Ansari, Niloufar; Crimeen-Irwin, Blessing; Nguyen, Huynh; Forwood, Mark R; Hu, Yifang; Ikegame, Mika; Bambery, Keith R; Petibois, Cyril; Mackie, Eleanor J; Tobin, Mark J; Smyth, Gordon K; Oakhill, Jonathan S; Martin, T John; Sims, Natalie A. Author Correction: Increased autophagy in EphrinB2-deficient osteocytes is associated with elevated secondary mineralization and brittle bone. Nature Communications. 2019;10(1):5073. 
- Comments: Used in: WB

Ye, Xiaoxia; Zhu, Mingming; Che, Xiaohang; Wang, Huiyang; Liang, Xing-Jie; Wu, Chunfu; Xue, Xue; Yang, Jingyu. Lipopolysaccharide induces neuroinflammation in microglia by activating the MTOR pathway and downregulating Vps34 to inhibit autophagosome formation. Journal Of Neuroinflammation. 2020;17(1):18. 
- Comments: Used in: ICC

Boukhalfa, Asma; Nascimbeni, Anna Chiara; Ramel, Damien; Dupont, Nicolas; Hirsch, Emilio; Gayral, Stephanie; Laffargue, Muriel; Codogno, Patrice; Morel, Etienne. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nature Communications. 2020;11(1):294. 
- Comments: Used in: ICC

Du, Ting-Ting; Zhu, Guanyu; Chen, Yingchuan; Shi, Lin; Liu, Defeng; Liu, Yuye; Zhang, Xin; Zhang, Jianguo. Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys. Aging. 2020;12(7):6324-6339. 
- Comments: Used in: WB

Rahaman, Md Shiblur; Banik, Subrata; Akter, Mahmuda; Rahman, Md Mostafizur; Sikder, Md Tajuddin; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki. Curcumin alleviates arsenic-induced toxicity in PC12 cells via modulating autophagy/apoptosis. Ecotoxicology And Environmental Safety. 2020;200:110756. 
- Comments: Used in: WB

Rahaman, Md Shiblur; Yamasaki, Shojiro; Binte Hossain, Kaniz Fatima; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki. Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells. Food And Chemical Toxicology : An International Journal Published For The British Industrial Biological Research Association. 2020;144:111577. 
- Comments: Used in: WB