Anti Keratan Sulfate (KS/Keratosulfate) lacking oversulfated structures mAb (Clone R-10G)

Cosmo Bio LTD

Catalog No: CAC-RIT-M001

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Application: IF, WB 
Clonality: Monoclonal 
Host: Mouse 
Purification: Ig-PG 
Reactivity: Human 

Monoclonal antibody R-10G recognizes keratan sulfate lacking oversulfated structures in oligosaccharide segments of keratan sulfate glycosaminoglycan chains on human induced pluripotent stem (hiPS) cells. Digestion with Keratanase II, Keratanase or Endo-beta-galactosidase removes these epitopes from keratan sulfate proteoglycans. Unlike commercially available keratan sulfate-recognizing antibodies 5D4 and BCD4, R-10G recognizes keratan sulfate with a low degree of sulfation. The antigenic polypeptide portion has been identified as podocalyxin. Importantly, R-10G antibody binds to human iPS cells and human ES cells, but only at very low levels to human embryonal carcinoma (EC) cells. Unlike widely used human pluripotent stem cell marker antibodies TRA-1-60 (IgM) and TRA-1-81 (IgM), R-10G (IgG1) binds only weakly to EC cells. Therefore, R-10G is useful for quality control of human iPS and ES cells.

References:
1) Kawabe K, et al, A novel antibody for human-induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures. Glycobiology. 2013 Mar;23(3):322-36. PubMed: 23154990
2) Schopperle WM, et al, The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007 Mar;25(3):723-30. PMID: 17124010

Citations for Anti Keratan Sulfate (KS/Keratosulfate) lacking oversulfated structures mAb (Clone R-10G) – 2 Found

Mawaribuchi et al. 2019. mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells. Sci Rep. 9:11910.   , Journal
- Comments: An ectoderm-biased substate of H9 human ES cells is characterized by low expression of the R-10G epitope and high expression of the mesenchymal marker vimentin.

Kawabe et al. 2013. A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.. Glycobiology. 23(3):322-336.   ,
- Comments: Initial description of R-10G monoclonal antibody