Anti INPP5D mAb (Clone 9G11)

CUSABIO

Catalog No.:
CSB-RA157463A0HU-50
Western Blot<br /> Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate<br /> All lanes: INPP5D antibody at 1:1500<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 134, 110 kDa<br /> Observed band size: 145 kDa<br />
IHC image of CSB-RA157463A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating SHIP in Raji whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA157463A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA157463A0HU(2µg)+ Raji whole cell lysate(500µg)<br /> Lane 3: Raji whole cell lysate (10µg)<br />
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Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 9G11)

Documents & Links for Anti INPP5D mAb (Clone 9G11)
Datasheet Anti INPP5D mAb (Clone 9G11) Datasheet

Documents & Links for Anti INPP5D mAb (Clone 9G11)
Datasheet Anti INPP5D mAb (Clone 9G11) Datasheet
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