Anti Importin-4 (IPO4) mAb (Clone 3C2)

Cosmo Bio LTD

Catalog No: CAC-CEC-023

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Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.

Application: ICC, WB, IF 
Clonality: Monoclonal 
Host: Rat 
Purification: Ion-exchange Chromatography 
Reactivity: Mouse, Rat, Human, Monkey, Hamster 

Background
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. [from: The UniProt Consortium, UniProt: a worldwide hub of protein knowledge, Nucleic Acids Research, Volume 47, Issue D1, 08 January 2019, Pages D506–D515, https://doi.org/10.1093/nar/gky1049]

Application
Western blotting : Recommended dilutions 1/1000 to 1/5000
Immunocytochemistry : Recommended dilutions 1/100 to 1/500

References
1) Miyauchi et al. (2005) J. Biol. Chem. 280:40901-40908.
2) Wang et al. (2010) PNAS. 107:16131-16136.