Anti ILK mAb (Clone 6A5)

CUSABIO

Catalog No.:
CSB-RA963626A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue<br /> All lanes: ILK antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 52, 45, 37 kDa<br /> Observed band size: 51 kDa<br />
Immunofluorescence staining of MCF7 Cells with CSB-RA963626A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ILK in Hela whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA963626A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA963626A0HU(2µg)+ Hela whole cell lysate(500µg)<br /> Lane 3: Hela whole cell lysate (10µg)<br />
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Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).

Product Specifications
Application WB, IF, IP, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 6A5)

Documents & Links for Anti ILK mAb (Clone 6A5)
Datasheet Anti ILK mAb (Clone 6A5) Datasheet

Documents & Links for Anti ILK mAb (Clone 6A5)
Datasheet Anti ILK mAb (Clone 6A5) Datasheet
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