Anti HSPB1 mAb (Clone 10C11)

CUSABIO

Catalog No.:
CSB-RA010833A0HU-100
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate, 293T whole cell lysate, LO2 whole cell lysate, HT-29 whole cell lysate, K562 whole cell lysate<br /> All lanes: Hsp27 antibody at 0.62μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 23 KDa<br /> Observed band size: 27 KDa<br />
IHC image of CSB-RA010833A0HU diluted at 1:61.9 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA010833A0HU diluted at 1:61.9 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-RA010833A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Product Specifications
Application WB, IHC, IF, IP, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 10C11)

Documents & Links for Anti HSPB1 mAb (Clone 10C11)
Datasheet Anti HSPB1 mAb (Clone 10C11) Datasheet

Documents & Links for Anti HSPB1 mAb (Clone 10C11)
Datasheet Anti HSPB1 mAb (Clone 10C11) Datasheet
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