Anti-HLA-E (Human) mAb (Clone: 4D12)

MBL Life Sciences

Catalog No.: MBL-K0215-3

HLA-E (human leucocyte antigen-E) is a conserved class I major histocompatibility molecule. It binds to the leader peptide derived from the polymorphic classical MHC molecules HLA-A, HLA-B and HLA-C. This peptide binding stabilizes the HLA-E protein and allows it to migrate to the cell surface. HLA-E then interacts with CD94/NKG2A receptors on natural killer cells. This interaction inhibits natural killer cell-mediated lysis of cells displaying HLA-E. In virally infected or tumor cells, down-regulation of HLA-A, HLA-B and HLA-C production prevents stabilization of HLA-E by the leader peptide. Under these circumstances, HLA-E is degraded before it reaches the cell surface and the cell is then vulnerable to lysis by natural killer cells. Monoclonal Antibody of 100 μl targeting HLA-E for FCM, IHC.

Product Specifications

• Application: FC, IHC
• Reactivity: Human
• Clonality: Monoclonal IgG1
• Host: Mouse
• Immunogen: Antibody obtained by immunizing HLA-B27 transgenic mice to HLA-E protein
• Formulation: 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Citations for Anti-HLA-E (Human) mAb (Clone: 4D12) – 3 Found

Nguyen, S; Beziat, V; Dhedin, N; Kuentz, M; Vernant, J P; Debre, P; Vieillard, V. HLA-E upregulation on IFN-gamma-activated AML blasts impairs CD94/NKG2A-dependent NK cytolysis after haplo-mismatched hematopoietic SCT. Bone Marrow Transplantation. 2009;43(9):693-9. 
- Comments: Used in: FCM

Tsukamoto, Nobuo; Okada, Starlyn; Onami, Yoko; Sasaki, Yusuke; Umezawa, Kazuo; Kawakami, Yutaka. Impairment of plasmacytoid dendritic cells for IFN production by the ligand for immunoglobulin-like transcript 7 expressed on human cancer cells. Clinical Cancer Research : An Official Journal Of The American Association For Cancer Research. 2009;15(18):5733-43. 
- Comments: Used in: FCM

Cui, Chang-Hao; Miyoshi, Shunichiro; Tsuji, Hiroko; Makino, Hatsune; Kanzaki, Seiichi; Kami, Daisuke; Terai, Masanori; Suzuki, Harumi; Umezawa, Akihiro. Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy. Human Molecular Genetics. 2011;20(2):235-44. 
- Comments: Used in: IHC