IHC image of CSB-RA010109A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Anti Histone H3.3 mAb (Clone 4H2)

Catalog No:
CSB-RA010109A0HU-50
$210.00
Application: IHC, IF, ELISA 
Clonality: Monoclonal 
Purification: Purified - Affinity 
Reactivity: Human 
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Documents & Links for Anti Histone H3.3 mAb (Clone 4H2)
DatasheetAnti Histone H3.3 mAb (Clone 4H2) Datasheet
Vendor PageAnti Histone H3.3 mAb (Clone 4H2) at CUSABIO

Documents & Links for Anti Histone H3.3 mAb (Clone 4H2)
DatasheetAnti Histone H3.3 mAb (Clone 4H2) Datasheet
Vendor PageAnti Histone H3.3 mAb (Clone 4H2)