Anti GDNF mAb (Clone 11D2)

CUSABIO

Catalog No.:
CSB-RA009356A0HU-100
Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate<br /> All lanes: GDNF antibody at 1μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 24, 21, 26, 23, 19 KDa<br /> Observed band size: 24 KDa<br />
IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating GDNF in Hela whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA009356A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA009356A0HU (3μg) + Hela whole cell lysate (500μg)<br /> Lane 3: Hela whole cell lysate (20μg)<br />
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Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Product Specifications
Application WB, IHC, IP, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 11D2)

Documents & Links for Anti GDNF mAb (Clone 11D2)
Datasheet Anti GDNF mAb (Clone 11D2) Datasheet

Documents & Links for Anti GDNF mAb (Clone 11D2)
Datasheet Anti GDNF mAb (Clone 11D2) Datasheet
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