Anti FEN1 mAb (Clone 4D9)

CUSABIO

Catalog No.:
CSB-RA008585A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate<br /> All lanes: FEN1 antibody at 0.775μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 43, 36 KDa<br /> Observed band size: 43 KDa<br />
IHC image of CSB-RA008585A0HU diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA008585A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 4D9)

Documents & Links for Anti FEN1 mAb (Clone 4D9)
Datasheet Anti FEN1 mAb (Clone 4D9) Datasheet

Documents & Links for Anti FEN1 mAb (Clone 4D9)
Datasheet Anti FEN1 mAb (Clone 4D9) Datasheet
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