Anti-Fas (CD95) mAb (Clone: CH-11)

MBL Life Sciences

Catalog No.: MBL-SY-001

Product Description

Monoclonal Antibody of 100 µl targeting Fas for FCM, WB.

Background

It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.

Source

This antibody was purified from ascites fluid using affinity chromatography. This hybridoma (clone CH-11) was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with the human diploid fibroblast FS-7 cell line.

Product Specifications

• Application: FC, WB
• Reactivity: Human
• Clonality: Monoclonal IgM
• Host: Mouse
• Immunogen: FS-7 Human diploid fibroblasts
• Gene ID - Human:

  355

• Gene ID - Mouse:

  14102

• Formulation: 0.5 mg/mL in PBS/50% glycerol, pH 7.2

Citations for Anti-Fas (CD95) mAb (Clone: CH-11) – 22 Found

Zanin-Zhorov, Alexandra; Hershkoviz, Rami; Hecht, Iris; Cahalon, Liora; Lider, Ofer. Fibronectin-associated Fas ligand rapidly induces opposing and time-dependent effects on the activation and apoptosis of T cells. Journal Of Immunology (Baltimore, Md. : 1950). 2003;171(11):5882-9. 
- Comments: Used in: Function

López-Huertas, María Rosa; Mateos, Elena; Sánchez Del Cojo, María; Gómez-Esquer, Francisco; Díaz-Gil, Gema; Rodríguez-Mora, Sara; López, Juan Antonio; Calvo, Enrique; López-Campos, Guillermo; Alcamí, José; Coiras, Mayte. The presence of HIV-1 Tat protein second exon delays fas protein-mediated apoptosis in CD4+ T lymphocytes: a potential mechanism for persistent viral production. The Journal Of Biological Chemistry. 2013;288(11):7626-7644. 
- Comments: Used in: Function

Murthy, Aditya; Li, Yun; Peng, Ivan; Reichelt, Mike; Katakam, Anand Kumar; Noubade, Rajkumar; Roose-Girma, Merone; DeVoss, Jason; Diehl, Lauri; Graham, Robert R; van Lookeren Campagne, Menno. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature. 2014;506(7489):456-62. 
- Comments: Used in: Function

Ma, Li; Gong, Haiyan; Zhu, Haiyan; Ji, Qing; Su, Pei; Liu, Peng; Cao, Shannan; Yao, Jianfeng; Jiang, Linlin; Han, Mingzhe; Ma, Xiaotong; Xiong, Dongsheng; Luo, Hongbo R; Wang, Fei; Zhou, Jiaxi; Xu, Yuanfu. A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model. The Journal Of Biological Chemistry. 2014;289(18):12457-66. 
- Comments: Used in: Function

Liu, Wei; Lin, Yan-Ting; Yan, Xiao-Li; Ding, Ya-Lan; Wu, Yun-Li; Chen, Wan-Nan; Lin, Xu. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology. 2015;29(3):1113-23. 
- Comments: Used in: Function

Yanagi, Teruki; Shi, Ranxin; Aza-Blanc, Pedro; Reed, John C; Matsuzawa, Shu-ichi. PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines. Plos One. 10(3):e0119404. 
- Comments: Used in: Function

Cui, X; Zhang, L; Magli, A R; Catera, R; Yan, X-J; Griffin, D O; Rothstein, T L; Barrientos, J; Kolitz, J E; Allen, S L; Rai, K R; Chiorazzi, N; Chu, C C. Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability. Leukemia. 2016;30(1):74-85. 
- Comments: Used in: Function

Tan, Lingbing; Zhang, Chaocan; Dematos, Julien; Kuang, Linlin; Jung, Jae U; Liang, Xiaozhen. CD95 Signaling Inhibits B Cell Receptor-Mediated Gammaherpesvirus Replication in Apoptosis-Resistant B Lymphoma Cells. Journal Of Virology. 2016;90(21):9782-9796. 
- Comments: Used in: Function

Hashiramoto, Akira; Konishi, Yoshitake; Murayama, Koichi; Kawasaki, Hiroki; Yoshida, Kohsuke; Tsumiyama, Ken; Tanaka, Kimie; Mizuhara, Masaru; Shiotsuki, Toshio; Kitamura, Hitomi; Komai, Koichiro; Kimura, Tomoatsu; Yagita, Hideo; Shiozawa, Kazuko; Shiozawa, Shunichi. A variant of death-receptor 3 associated with rheumatoid arthritis interferes with apoptosis-induction of T cell. The Journal Of Biological Chemistry. 2018;293(6):1933-1943. 
- Comments: Used in: Function

Takaki, Syohei; Eto, Ko. Cytoplasmic localization of programmed cell death 4 contributes to its anti-apoptotic function. Molecular And Cellular Biochemistry. 2018;448(1-2):155-164. 
- Comments: Used in: NT

Kozako, Tomohiro; Mellini, Paolo; Ohsugi, Takeo; Aikawa, Akiyoshi; Uchida, Yu-Ichiro; Honda, Shin-Ichiro; Suzuki, Takayoshi. Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines. Bmc Cancer. 2018;18(1):791. 
- Comments: Used in: Function

Jing, Zhen-Tang; Liu, Wei; Wu, Shu-Xiang; He, Yun; Lin, Yan-Ting; Chen, Wan-Nan; Lin, Xin-Jian; Lin, Xu. Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation. Journal Of Immunology (Baltimore, Md. : 1950). 2018;201(8):2303-2314. 
- Comments: Used in: Function

Sekiguchi, Yuto; Yamada, Mayuka; Noguchi, Takuya; Noomote, Chise; Tsuchida, Mei; Kudoh, Yuki; Hirata, Yusuke; Matsuzawa, Atsushi. The anti-cancer drug gefitinib accelerates Fas-mediated apoptosis by enhancing caspase-8 activation in cancer cells. The Journal Of Toxicological Sciences. 44(6):435-440. 
- Comments: Used in: Function

Liu, Wei; Jing, Zhen-Tang; Xue, Chao-Rong; Wu, Shu-Xiang; Chen, Wan-Nan; Lin, Xin-Jian; Lin, Xu. PI3K/AKT inhibitors aggravate death receptor-mediated hepatocyte apoptosis and liver injury. Toxicology And Applied Pharmacology. 2019;381:114729. 
- Comments: Used in: Function

Dagenais-Lussier, Xavier; Loucif, Hamza; Cadorel, Hugo; Blumberger, Juliette; Isnard, Stéphane; Bego, Mariana Gé; Cohen, Éric A; Routy, Jean-Pierre; van Grevenynghe, Julien. USP18 is a significant driver of memory CD4 T-cell reduced viability caused by type I IFN signaling during primary HIV-1 infection. Plos Pathogens. 2019;15(10):e1008060. 
- Comments: Used in: Function

Asakawa, Masahiro; Itoh, Michiko; Suganami, Takayoshi; Sakai, Takeru; Kanai, Sayaka; Shirakawa, Ibuki; Yuan, Xunmei; Hatayama, Tomomi; Shimada, Shu; Akiyama, Yoshimitsu; Fujiu, Katsuhito; Inagaki, Yutaka; Manabe, Ichiro; Yamaoka, Shoji; Yamada, Tetsuya; Tanaka, Shinji; Ogawa, Yoshihiro. Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis. Scientific Reports. 2019;9(1):19601. 
- Comments: Used in: Function

Masuda, Azuchi; Isobe, Yasushi; Sugimoto, Koichi; Yoshimori, Mayumi; Arai, Ayako; Komatsu, Norio. Efficient recruitment of c-FLIP(L) to the death-inducing signaling complex leads to Fas resistance in natural killer-cell lymphoma. Cancer Science. 2020;111(3):807-816. 
- Comments: Used in: WB

Masuda, Azuchi; Isobe, Yasushi; Sugimoto, Koichi; Yoshimori, Mayumi; Arai, Ayako; Komatsu, Norio. Efficient recruitment of c-FLIP(L) to the death-inducing signaling complex leads to Fas resistance in natural killer-cell lymphoma. Cancer Science. 2020;111(3):807-816. 
- Comments: Used in: Function

Aoki, Kana; Satoi, Shinsuke; Harada, Shota; Uchida, Seiichi; Iwasa, Yoh; Ikenouchi, Junichi. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Molecular Biology Of The Cell. 2020;31(8):833-844. 
- Comments: Used in: Function

Thiagarajan, D; Fiskesund, R; Frostegård, A; Steen, J; Rahman, M; Vikström, M; Lundström, S; Frostegård, J. Immunoglobulin G1 Antibodies Against Phosphorylcholine Are Associated With Protection in Systemic Lupus Erythematosus and Atherosclerosis: Potential Underlying Mechanisms. Acr Open Rheumatology. 2020;2(6):344-356. 
- Comments: Used in: Function

Graeter, Stefanie; Schneider, Christoph; Verschoor, Daniëlle; von Däniken, Sandro; Seibold, Frank; Yawalkar, Nikhil; Villiger, Peter; Dimitrov, Jordan D; Smith, David F; Cummings, Richard D; Simon, Hans-Uwe; Vassilev, Tchavdar; von Gunten, Stephan. Enhanced Pro-apoptotic Effects of Fe(II)-Modified IVIG on Human Neutrophils. Frontiers In Immunology. 11:973. 
- Comments: Used in: Function

Liu, Dan; Wang, Zhiding; Wang, Huijuan; Ren, Feifei; Li, Yanqin; Zou, Sifan; Xu, Jianqiao; Xie, Lixin. The protective role of miR-223 in sepsis-induced mortality. Scientific Reports. 2020;10(1):17691. 
- Comments: Used in: Function