Anti ELANE mAb (Clone 2H12)

CUSABIO

SKU:
CSB-RA200985A0HU-50
  • IHC image of CSB-RA200985A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing Jurkat cells stained with CSB-RA200985A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Product Specifications
Application IHC, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2H12)


Documents & Links for Anti ELANE mAb (Clone 2H12)
Datasheet Anti ELANE mAb (Clone 2H12) Datasheet

Documents & Links for Anti ELANE mAb (Clone 2H12)
Datasheet Anti ELANE mAb (Clone 2H12) Datasheet



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