Anti DNM2 mAb (Clone 1D8)

CUSABIO

SKU:
CSB-RA877222A0HU-50
  • Western Blot<br /> Positive WB detected in: HL60 whole cell lysate, Rat brain tissue<br /> All lanes: DNM2 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 99, 98 kDa<br /> Observed band size: 99 kDa<br />
  • Immunofluorescence staining of Hela Cells with CSB-RA877222A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Overlay histogram showing Hela cells stained with CSB-RA877222A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:12498685). Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity).

Product Specifications
Application WB, IF, FC, ELISA
Reactivity Human, Rat
Clonality Monoclonal (Clone No.: 1D8)


Documents & Links for Anti DNM2 mAb (Clone 1D8)
Datasheet Anti DNM2 mAb (Clone 1D8) Datasheet

Documents & Links for Anti DNM2 mAb (Clone 1D8)
Datasheet Anti DNM2 mAb (Clone 1D8) Datasheet



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