Anti Di-methyl-Histone H3.1 (K4) mAb (Clone 6F6)

CUSABIO

Catalog No.:
CSB-RA010418A04me2HU-50
Western Blot<br /> Positive WB detected in Mouse brain tissue,Mouse heart tissue<br /> All lanes Di-methyl-Histone H3.1(K4)antibody at 0.55μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 15 KDa<br /> Observed band size: 15 KDa<br />
Immunocytochemistry analysis of CSB-RA010418A04me2HU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA010418A04me2HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA010418A04me2HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product Specifications
Application WB, ICC, IF, FC, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 6F6)

Documents & Links for Anti Di-methyl-Histone H3.1 (K4) mAb (Clone 6F6)
Datasheet Anti Di-methyl-Histone H3.1 (K4) mAb (Clone 6F6) Datasheet

Documents & Links for Anti Di-methyl-Histone H3.1 (K4) mAb (Clone 6F6)
Datasheet Anti Di-methyl-Histone H3.1 (K4) mAb (Clone 6F6) Datasheet
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