Anti DHFR mAb (Clone 9B2)

CUSABIO

Catalog No.:
CSB-RA264582A0HU-100
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue<br /> All lanes: DHFR antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 22, 16 kDa<br /> Observed band size: 22 kDa<br />
IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating DHFR in Hela whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA264582A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA264582A0HU(2µg)+ Hela whole cell lysate(500µg)<br /> Lane 3: Hela whole cell lysate (10µg)<br />
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Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human, Mouse, Rat
Clonality Monoclonal (Clone No.: 9B2)

Documents & Links for Anti DHFR mAb (Clone 9B2)
Datasheet Anti DHFR mAb (Clone 9B2) Datasheet

Documents & Links for Anti DHFR mAb (Clone 9B2)
Datasheet Anti DHFR mAb (Clone 9B2) Datasheet
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