Anti CYP19A1 mAb (Clone 1H1)

CUSABIO

Catalog No.:
CSB-RA006394A0HU-50
Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate<br /> All lanes: CYP19A1 antibody at 2.3μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 58, 25 KDa<br /> Observed band size: 58 KDa<br />
IHC image of CSB-RA006394A0HU diluted at 1:230 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-RA006394A0HU at 1:76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Catalyzes the formation of aromatic C18 estrogens from C19 androgens.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1H1)

Documents & Links for Anti CYP19A1 mAb (Clone 1H1)
Datasheet Anti CYP19A1 mAb (Clone 1H1) Datasheet

Documents & Links for Anti CYP19A1 mAb (Clone 1H1)
Datasheet Anti CYP19A1 mAb (Clone 1H1) Datasheet
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