Anti CYP17A1 mAb (Clone 5A9)

CUSABIO

Catalog No.:
CSB-RA554990A0HU-50
Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate<br /> All lanes: CYP17A1 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 58 kDa<br /> Observed band size: 58 kDa<br />
IHC image of CSB-RA554990A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA554990A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human, Rat
Clonality Monoclonal (Clone No.: 5A9)

Documents & Links for Anti CYP17A1 mAb (Clone 5A9)
Datasheet Anti CYP17A1 mAb (Clone 5A9) Datasheet

Documents & Links for Anti CYP17A1 mAb (Clone 5A9)
Datasheet Anti CYP17A1 mAb (Clone 5A9) Datasheet
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