Anti Collagen Alpha-1(VII) Chain mAb (Clone BML39)

Cosmo Bio

SKU:
CAC-NU-01-CO7
  • Figure 1. Western Blotting.

Concentrated conditioned medium prepared from BMGE+H cells was immunoblotted with BML39 antibody (1:50 dilution). BML39 antibody detected type-7 collagen as a band at approximately 300 kDa (full-length) and degraded fragments around 170 kDa Conditioned medium was concentrated by ammonia sulfate precipitation as described previously (ref. 2). Polypeptides were separated by SDS-PAGE (7.5%).
  • Figure 2. Immunofluorescence microscopy of human skin and rabbit large intestine.Frozen sections of human skin (left) and rabbit large intestine (right) were stained with BML39 antibody at 1:200 dilution. The antibody revealed the location of type VII collagen at basement membranes. Bars = 40 um. Frozen sections were prepared as described previously (ref. 2).
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This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. The inherited disease, dystrophic epidermolysis bullosa, is caused by recessive or dominant mutations in COL7A1. In the absence of mutations, however, an autoimmune response against type VII collagen can result in an acquired form of this disease called epidermolysis bullosa acquisita. Type VII collagen is also found in the retina; its function in this organ is unknown. Collagen, type VII, alpha 1 has been shown to interact with Laminin 5 and Fibronectin.

Source: Nagoya University Graduate School of Science Department of Life Science Cell Biology Group Lecturer Mr. Yoshiko Hirako

References:
1) Uematsu J., et al. Eur J Cell Biol., 84: 407-415 (2005).
2) Hirako Y., et al. J. Biol. Chem., 273:9711-9717 (1998).



Product Specifications
Application IP, IF, WB, IHC(f)
Reactivity Porcine, Rabbit, Bovine, Human
Clonality Monoclonal (Clone No.: BML39)
Host Mouse


Documents & Links for Anti Collagen Alpha-1(VII) Chain mAb (Clone BML39)
Datasheet cac-nu-01-co7_anti-collagen-7-mab-clone-bml39_datasheet.pdf

Documents & Links for Anti Collagen Alpha-1(VII) Chain mAb (Clone BML39)
Datasheet cac-nu-01-co7_anti-collagen-7-mab-clone-bml39_datasheet.pdf