Anti CDKN1B mAb (Clone 1G2)

CUSABIO

Catalog No.:
CSB-RA005087A0HU-50
Western Blot<br /> Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate<br /> All lanes: CDKN1B antibody at 0.9μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 23 KDa<br /> Observed band size: 27 KDa<br />
IHC image of CSB-RA005087A0HU diluted at 1:97.5 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA005087A0HU diluted at 1:97.5 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-RA005087A0HU at 1:32.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 1G2)

Documents & Links for Anti CDKN1B mAb (Clone 1G2)
Datasheet Anti CDKN1B mAb (Clone 1G2) Datasheet

Documents & Links for Anti CDKN1B mAb (Clone 1G2)
Datasheet Anti CDKN1B mAb (Clone 1G2) Datasheet
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