Anti CDK4 mAb (Clone 8F2)

CUSABIO

Catalog No.:
CSB-RA965615A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate<br /> All lanes: CDK4 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 34, 21 kDa<br /> Observed band size: 34 kDa<br />
Immunoprecipitating CDK4 in Hela whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA965615A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)<br /> Lane 2:CSB-RA965615A0HU(2µg)+ Hela whole cell lysate(500µg)<br /> Lane 3: Hela whole cell lysate (10µg)<br />
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Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Product Specifications
Application WB, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 8F2)

Documents & Links for Anti CDK4 mAb (Clone 8F2)
Datasheet Anti CDK4 mAb (Clone 8F2) Datasheet

Documents & Links for Anti CDK4 mAb (Clone 8F2)
Datasheet Anti CDK4 mAb (Clone 8F2) Datasheet
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