Anti CD99 mAb (Clone 9A5)

CUSABIO

Catalog No.:
CSB-RA004973A0HU-50
Western Blot<br /> Positive WB detected in Jurkat whole cell lysate<br /> All lanes CD99 antibody at 0.8μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 28 KDa<br /> Observed band size: 28 KDa<br />
IHC image of CSB-RA004973A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Overlay histogram showing Jurkat cells stained with CSB-RA004973A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Product Specifications
Application WB, IHC, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 9A5)

Documents & Links for Anti CD99 mAb (Clone 9A5)
Datasheet Anti CD99 mAb (Clone 9A5) Datasheet

Documents & Links for Anti CD99 mAb (Clone 9A5)
Datasheet Anti CD99 mAb (Clone 9A5) Datasheet
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