Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12, Biotin Labeled)

Cosmo Bio

SKU:
CAC-SHI-EXO-M01-B
  • Figure 1. Generic exosome shown with commonly associated molecules. Not all molecules will be found with any given exosome and other molecules may also be found.
  • Figure 2. Western blot analysis of CD9 in C32 melanoma cell exosomes using anti-CD9 (12A12) antibody. Sample: C32 cell Exosome (5ug, 1ug) Primary antibody: anti-CD9 (12A12) (1ug/mL) Secondary antibody: anti-mouse IgG-HRP
  • Figure 3. IP-WB of human serum exosomes. Sample 1. Anti-CD9 (12A12) - Serum A 2. Control antibody - Serum A 3. Anti-CD9 (12A12) - Serum B 4. Control antibody - Serum B 5. Ultracentrifugation - Serum A 6. Ultracentrifugation - Serum A
  • Figure 4. ELISA of C32 melanoma cell exosomes with anti-CD9 (12A12-ALP) antibody (5 ng/mL).
  • Figure 5. CD9 and CD63 molecules on the surface of extracellular vesicles from human breast cancer cell line (MDA-MB-231-luc-D3H2LN)) were detected by anti-human CD9 antibody (SHI-EXO-M01) and anti-human CD63 antibody (SHI-EXO-M02).
Shipping:
Calculated at Checkout
$935.00
Adding to cart… The item has been added

Click here for more information and to see all exosome related products from Cosmo Bio USA.

CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD9 is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. Importantly, it is found on the surface of exosomes.

Exosomes are cell-derived vesicles bounded by a lipid bilayer membrane and exhibiting a diameter of 50 to 150 nm. They are secreted from cultured cells and are observed in body fluids such as saliva, blood, urine, amniotic fluid, malignant ascites. Recent studies indicate that exosomes contain various proteins and RNAs, suggesting a role in information transfer between cells.

This monoclonal antibody can be used to immunoprecipitate exosomes from serum and culture supernatants.

References:
1) Shigeyasu Tsuda et al., Scientific Reports volume 7, Article number: 12989 (2017)
2) N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
3) Matsuzaki K et al., Oncotarget. 2017 Apr 11; 8(15): 24668–24678. doi: 10.18632/oncotarget.14969
4) Kazutoshi Fujita et al., Sci Rep. 2017; 7: 42961. doi: 10.1038/srep42961
5) Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
6) Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.
7) Yagi Y et al., Neurosci Lett. 2017 Jan 1;636:48-57. doi: 10.1016/j.neulet.2016.10.042. Epub 2016 Oct 22.
8) Ueda K et al., Sci Rep. 2014 Aug 29;4:6232. doi: 10.1038/srep06232.



Product Specifications
Application ELISA, IP, WB
Reactivity Human
Clonality Monoclonal (Clone No.: 12A12)
Host Mouse
Conjugation Biotin


Documents & Links for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12, Biotin Labeled)
Datasheet Anti CD9 mAb (Clone 12A12, Biotin Labeled) Datasheet

Documents & Links for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12, Biotin Labeled)
Datasheet Anti CD9 mAb (Clone 12A12, Biotin Labeled) Datasheet



Citations for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12, Biotin Labeled) – 1 Found
Masaki, Kanako; Ahmed, Abo Bakr F; Ishida, Takenori; Mikami, Yuuki; Funabashi, Hisakage; Hirota, Ryuichi; Ikeda, Takeshi; Kuroda, Akio. Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes. Biotechnology Letters. 2023;45(11-12):1457-1466.  PubMed
!