Anti CD8A mAb (Clone 2F2)

CUSABIO

Catalog No.:
CSB-RA934881A0HU-50
Western Blot<br /> Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate<br /> All lanes: CD8A antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 26, 22, 31 kDa<br /> Observed band size: 31 kDa<br />
IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD8A in HepG2 whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA934881A0HU in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2:CSB-RA934881A0HU(3µg)+ HepG2 whole cell lysate(500µg)<br /> Lane 3: HepG2 whole cell lysate (10µg)<br />
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Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2F2)

Documents & Links for Anti CD8A mAb (Clone 2F2)
Datasheet Anti CD8A mAb (Clone 2F2) Datasheet

Documents & Links for Anti CD8A mAb (Clone 2F2)
Datasheet Anti CD8A mAb (Clone 2F2) Datasheet
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