Anti CD80 mAb (Clone 3C3)

CUSABIO

Catalog No.:
CSB-RA246383A0HU-50
Western Blot<br /> Positive WB detected in: Raji whole cell lysate<br /> All lanes: CD80 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 34, 30, 19 kDa<br /> Observed band size: 60 kDa<br />
IHC image of CSB-RA246383A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD80 in Raji whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA246383A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA246383A0HU(2µg)+ Raji whole cell lysate(500µg)<br /> Lane 3: Raji whole cell lysate (10µg)<br />
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Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Product Specifications
Application WB, IHC, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 3C3)

Documents & Links for Anti CD80 mAb (Clone 3C3)
Datasheet Anti CD80 mAb (Clone 3C3) Datasheet

Documents & Links for Anti CD80 mAb (Clone 3C3)
Datasheet Anti CD80 mAb (Clone 3C3) Datasheet
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