Anti CD21 mAb (Clone 16F10)

CUSABIO

Catalog No.:
CSB-RA005934A0HU-50
Western Blot<br /> Positive WB detected in Raji whole cell lysate<br /> All lanes CD21 antibody at 0.55μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 155 KDa<br /> Observed band size: 155 KDa<br />
IHC image of CY5710 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA005934A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Raji cells with CSB-RA005934A0HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

Product Specifications
Application WB, IHC, IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 16F10)

Documents & Links for Anti CD21 mAb (Clone 16F10)
Datasheet Anti CD21 mAb (Clone 16F10) Datasheet

Documents & Links for Anti CD21 mAb (Clone 16F10)
Datasheet Anti CD21 mAb (Clone 16F10) Datasheet
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