Anti CD146 mAb (Clone 8A10)

CUSABIO

SKU:
CSB-RA013563A0HU-50
  • Western Blot<br /> Positive WB detected in A375 whole cell lysate<br /> All lanes CD146 antibody at 0.6μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 72 KDa<br /> Observed band size: 120 KDa<br />
  • IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Overlay histogram showing A375 cells stained with CSB-RA013563A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Product Specifications
Application WB, IHC, IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 8A10)


Documents & Links for Anti CD146 mAb (Clone 8A10)
Datasheet Anti CD146 mAb (Clone 8A10) Datasheet

Documents & Links for Anti CD146 mAb (Clone 8A10)
Datasheet Anti CD146 mAb (Clone 8A10) Datasheet



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