Anti CCNE1 mAb (Clone 3A5)

CUSABIO

Catalog No.:
CSB-RA968740A0HU-50
IHC image of CSB-RA968740A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA968740A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA968740A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Essential for the control of the cell cycle at the G1/S (start) transition.

Product Specifications
Application IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 3A5)

Documents & Links for Anti CCNE1 mAb (Clone 3A5)
Datasheet Anti CCNE1 mAb (Clone 3A5) Datasheet

Documents & Links for Anti CCNE1 mAb (Clone 3A5)
Datasheet Anti CCNE1 mAb (Clone 3A5) Datasheet
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