Anti CASP9 mAb (Clone 2D5)

CUSABIO

Catalog No.:
CSB-RA004555A0HU-50
Western Blot<br /> Positive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysate<br /> All lanes: CASP9 antibody at 1.8μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 47, 31, 18, 37 KDa<br /> Observed band size: 47 KDa<br />
Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Shipping:
Calculated at Checkout
$221.00
Adding to cart… The item has been added
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Product Specifications
Application WB, IF, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 2D5)

Documents & Links for Anti CASP9 mAb (Clone 2D5)
Datasheet Anti CASP9 mAb (Clone 2D5) Datasheet

Documents & Links for Anti CASP9 mAb (Clone 2D5)
Datasheet Anti CASP9 mAb (Clone 2D5) Datasheet
!