Anti CASP3 mAb (Clone 5B2)

CUSABIO

Catalog No.:
CSB-RA286668A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate<br /> All lanes: pro Caspase 3 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 32 kDa<br /> Observed band size: 32 kDa<br />
IHC image of CSB-RA286668A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA286668A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Product Specifications
Application WB, IHC, IF, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 5B2)

Documents & Links for Anti CASP3 mAb (Clone 5B2)
Datasheet Anti CASP3 mAb (Clone 5B2) Datasheet

Documents & Links for Anti CASP3 mAb (Clone 5B2)
Datasheet Anti CASP3 mAb (Clone 5B2) Datasheet
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