Anti C3 mAb (Clone 4D12)

CUSABIO

SKU:
CSB-RA303909A0HU-50
  • Western Blot<br /> Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate<br /> All lanes: C3 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 188 kDa<br /> Observed band size: 115 kDa<br />
  • IHC image of CSB-RA303909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing HepG2 cells stained with CSB-RA303909A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Product Specifications
Application WB, IHC, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 4D12)


Documents & Links for Anti C3 mAb (Clone 4D12)
Datasheet Anti C3 mAb (Clone 4D12) Datasheet

Documents & Links for Anti C3 mAb (Clone 4D12)
Datasheet Anti C3 mAb (Clone 4D12) Datasheet



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