![Example Assay Data: 1. Sample Preparation. Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0) Protein was expressed in E.coli and lysed (Crude sample). Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting. 2. Immunoblotting analysis. Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL) Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag: Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/1280x1280/products/29850/120401/cac-xim-ma001_anti-c-myc-mab-clone-9e10_7785__14705.1620917195.png?c=2 "Example Assay Data: 1. Sample Preparation. Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0) Protein was expressed in E.coli and lysed (Crude sample). Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting. 2. Immunoblotting analysis. Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL) Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag: Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.")
![Example Assay Data: 1. Sample Preparation. Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0) Protein was expressed in E.coli and lysed (Crude sample). Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting. 2. Immunoblotting analysis. Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL) Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag: Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/500x659/products/29850/120401/cac-xim-ma001_anti-c-myc-mab-clone-9e10_7785__14705.1620917195.png?c=2 "Example Assay Data: 1. Sample Preparation. Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0) Protein was expressed in E.coli and lysed (Crude sample). Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting. 2. Immunoblotting analysis. Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL) Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag: Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.")
Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.
Application: FC, IP, IHC, WB, ChIP, ELISAClonality: Monoclonal
Host: Mouse
Purification: Ig-PG
Reactivity: Human
c-Myc is a very potent oncogene upregulated in various types of cancer. c-Myc, through its role as a transcription factor, alters the expression of hundreds of target genes, often oncogenes or tumor suppressors. Clone 9E10 recognizes recombinant proteins that incorporate the c-Myc epitope tag and detects human c-Myc proteins and peptides in random coil rather than alpha-helix structures.
References:
1) Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960. (WB)
2) Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842. (ChIP)
3) Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916. (IP, WB)
4) Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822. (IP, WB)
5) Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423. (IHC)
6) Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455. (IHC)
7) Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969. (IP)
8) Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782. (WB)
| Documents & Links for Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10) | |
| Datasheet | Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10) Datasheet |
| Documents & Links for Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10) | |
| Datasheet | Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10) Datasheet |