Anti BUB1B mAb (Clone 7H4)

CUSABIO

Catalog No.:
CSB-RA263021A0HU-100
Western Blot<br /> Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate<br /> All lanes: BubR1 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 120, 106, 122 kDa<br /> Observed band size: 125 kDa<br />
Overlay histogram showing Hela cells stained with CSB-RA263021A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Product Specifications
Application WB, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 7H4)

Documents & Links for Anti BUB1B mAb (Clone 7H4)
Datasheet Anti BUB1B mAb (Clone 7H4) Datasheet

Documents & Links for Anti BUB1B mAb (Clone 7H4)
Datasheet Anti BUB1B mAb (Clone 7H4) Datasheet
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