Anti BRD4 mAb (Clone CL1115, ATL-AMAb90841 w/enhanced validation)

Atlas Antibodies

SKU:
ATL-AMAb90841-25
  • Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
  • Immunofluorescence staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
  • Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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Protein Description: bromodomain containing 4
Gene Name: BRD4
Alternative Gene Name: CAP, HUNK1, HUNKI, MCAP
Clone ID: CL1115
Isotype: IgG1
Interspecies mouse/rat: ENSMUSG00000024002: 76%, ENSRNOG00000006770: 76%
Entrez Gene ID: 23476
Uniprot ID: O60885
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Storage Temperature: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Product Specifications
Application WB, ICC, IHC
Reactivity Human
Clonality Monoclonal IgG1 (Clone No.: CL1115)
Host Mouse
Gene ID - Mouse ENSMUSG00000024002
Gene ID - Rat ENSRNOG00000006770
Buffer 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.



Documents & Links for Anti BRD4 mAb (Clone CL1115, ATL-AMAb90841 w/enhanced validation)
Datasheet Anti BRD4 mAb (Clone CL1115, ATL-AMAb90841 w/enhanced validation) Datasheet (External Link)
Vendor Page Anti BRD4 mAb (Clone CL1115, ATL-AMAb90841 w/enhanced validation)



Citations for Anti BRD4 mAb (Clone CL1115, ATL-AMAb90841 w/enhanced validation) – 3 Found
Luo, Xin; Archibeque, Ivonne; Dellamaggiore, Ken; Smither, Kate; Homann, Oliver; Lipford, James Russell; Mohl, Dane. Profiling of diverse tumor types establishes the broad utility of VHL-based ProTaCs and triages candidate ubiquitin ligases. Iscience. 2022;25(3):103985.  PubMed
Kitagawa, Takao; Kobayashi, Daiki; Baron, Byron; Okita, Hajime; Miyamoto, Tatsuo; Takai, Rie; Paudel, Durga; Ohta, Tohru; Asaoka, Yoichi; Tokunaga, Masayuki; Nakagawa, Koji; Furutani-Seiki, Makoto; Araki, Norie; Kuramitsu, Yasuhiro; Kobayashi, Masanobu. AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells. Plos One. 17(10):e0269077.  PubMed
Singh, Amit K; Chen, Qingrong; Nguyen, Cu; Meerzaman, Daoud; Singer, Dinah S. Cohesin regulates alternative splicing. Science Advances. 2023;9(9):eade3876.  PubMed