Anti BPAG1 (BP230)

Cosmo Bio

Catalog No.:
CAC-NU-01-BP1
Figure 1. WB analysis. Whole cell lysate prepared from DJM-1 cells was immunoblotted with mAb 279 at 1:100 dilution. mAb 279 recognized BPAG1 (Bullous Pemphigoid antigen 1) as a band at approximately 230 kDa (arrow).
Figure 2. Immunofluorescence microscopy of human skin. Human skin was stained by mAb 279 (1:50), showing that BPAG1 localized at the dermal -epidermal junction (green). Bar: 40 um.
Figure 3. Immunofluorescence microscopy of bovine skin. Bovine skin was stained by mAb 279 (1:50 dilution). Myoepithelial cells of skin apocrine glands were demonstrated to express BPAG1 (left panel, green). The close-up image (right) shows dotted lines of hemidesmosomes, which are cell to substrate adhesion structures containing BPAG1. Scale bar: 20 um.
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Product Description

Hemidesmosomes are adhesive structures between cells and the extracellular matrix. They play a role in anchoring intermediate fibers to the extracellular basement membrane. Structurally, hemidesmosomes occur in two forms: Type I and Type II. Type I hemidesmosomes develop in stratified epithelia such as the epidermis. Its main components include the intracellular linker proteins Plectin and BPAG1, the adhesion receptor integrin α6β4 and collagen type BP180/XVII. Type II hemidesmosomes occur in blood vessels, Schwann cells, and digestive tract epithelia as a simplified form of Type I hemidesmosomes, consisting only plectin and integrin α6β4. The hemidesmosomal adhesion receptor is normally associated with Laminin 5 in the basement membrane. Furthermore, Laminin 5 (of which Laminin gamma 2 is a subunit) is linked to collagen fibers in the dermis via type VII collagen. Genetic deletion of hemidesmosome-related proteins causes various forms of epidermolysis bullosa, highlighting their importance in promoting adhesion between the epidermis and the basement membrane.

BPAG1 is a 230-kDa cytoplasmic protein localized to hemidesmosomes of stratified epithelia and is one of the major antigenic proteins of bullous pemphigoid (BP), a chronic autoimmune skin disease. This anti-BPAG1 monoclonal antibody (clone 279) was raised against bovine BPAG1 and can be used in western blotting and immunofluorescent staining (IF) in multiple animal species, including humans and mice.

References:


1) Hirako Y., et al. Exp. Cell Res., 324: 172-182 (2014).
2) Okumura M., et al. J Biol Chem., 277:6682-6687 (2002)
3) Hirako Y., et al., J Biol Chem., 273:9711-9717 (1998)



Product Specifications
Application IF, WB, IHC(f)
Reactivity Porcine, Rabbit, Bovine, Rat, Human
Clonality Monoclonal (Clone No.: 279)
Host Mouse

Documents & Links for Anti BPAG1 (BP230)
Datasheet Anti BPAG1 (BP230) Datasheet

Documents & Links for Anti BPAG1 (BP230)
Datasheet Anti BPAG1 (BP230) Datasheet


Documentation Requirements for Order Placement

Material Transfer or Licensing Agreement (MTA)
The manufacturer of this product requires a signed Material Transfer or Licensing Agreement to be submitted at time of order placement. Access the agreement in the Documents section below. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.

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