Anti BMI1 mAb (Clone 1F2)

CUSABIO

Catalog No.:
CSB-RA916472A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate<br /> All lanes: BMI1 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 37 kDa<br /> Observed band size: 45 kDa<br />
IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 1F2)

Documents & Links for Anti BMI1 mAb (Clone 1F2)
Datasheet Anti BMI1 mAb (Clone 1F2) Datasheet

Documents & Links for Anti BMI1 mAb (Clone 1F2)
Datasheet Anti BMI1 mAb (Clone 1F2) Datasheet
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