Anti BCL2 mAb (Clone 3F6)

CUSABIO

Catalog No.:
CSB-RA564360A0HU-100
Western Blot<br /> Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U-937 whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate, Mouse Spleen whole cell lysate<br /> All lanes: Bcl-2 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 27, 23 kDa<br /> Observed band size: 27 kDa<br />
IHC image of CSB-RA564360A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Product Specifications
Application WB, IHC, ELISA
Reactivity Human, Mouse
Clonality Monoclonal (Clone No.: 3F6)

Documents & Links for Anti BCL2 mAb (Clone 3F6)
Datasheet Anti BCL2 mAb (Clone 3F6) Datasheet

Documents & Links for Anti BCL2 mAb (Clone 3F6)
Datasheet Anti BCL2 mAb (Clone 3F6) Datasheet
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