Anti BAK1 mAb (Clone 8D1)

CUSABIO

Catalog No.:
CSB-RA624111A0HU-50
Western Blot<br /> Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate<br /> All lanes: BAK1 antibody at 0.9μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 24, 17 KDa<br /> Observed band size: 24 KDa<br />
IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating BAK1 in HEK293 whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA624111A0HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA624111A0HU (3μg) + HEK293 whole cell lysate (500μg)<br /> Lane 3: HEK293 whole cell lysate (20μg)<br />
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Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

Product Specifications
Application WB, IHC, IP, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 8D1)

Documents & Links for Anti BAK1 mAb (Clone 8D1)
Datasheet Anti BAK1 mAb (Clone 8D1) Datasheet

Documents & Links for Anti BAK1 mAb (Clone 8D1)
Datasheet Anti BAK1 mAb (Clone 8D1) Datasheet
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