Anti AR mAb (Clone 5G7)

CUSABIO

SKU:
CSB-RA217661A0HU-50
  • Western Blot<br />Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate<br />All lanes: Androgen Receptor antibody at 1:1000<br />Secondary<br />Goat polyclonal to rabbit IgG at 1/50000 dilution<br />Predicted band size: 100, 45, 68, kDa<br />Observed band size: 125 kDa<br />
  • IHC image of CSB-RA217661A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA217661A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Product Specifications
Application WB, IHC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 5G7)


Documents & Links for Anti AR mAb (Clone 5G7)
Datasheet Anti AR mAb (Clone 5G7) Datasheet

Documents & Links for Anti AR mAb (Clone 5G7)
Datasheet Anti AR mAb (Clone 5G7) Datasheet



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