Anti APOA2 mAb (Clone 6H2)

CUSABIO

Catalog No.:
CSB-RA001915A0HU-50
Western Blot<br /> Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate<br /> All lanes: Apolipoprotein A II antibody at 0.87μg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 12 KDa<br /> Observed band size: 12 KDa<br />
IHC image of CSB-RA001915A0HU diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Overlay histogram showing A549 cells stained with CSB-RA001915A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

Product Specifications
Application WB, IHC, FC, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 6H2)

Documents & Links for Anti APOA2 mAb (Clone 6H2)
Datasheet Anti APOA2 mAb (Clone 6H2) Datasheet

Documents & Links for Anti APOA2 mAb (Clone 6H2)
Datasheet Anti APOA2 mAb (Clone 6H2) Datasheet
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